Sensing scaffolds to monitor cellular activity using impedance measurements

Yudan Whulanza, Nadia Ucciferri, Claudio Domenici, Giovanni Vozzi, Arti Ahluwalia

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)

Abstract

Scaffolds are cell adhesive matrices for the realisation of tissue constructs. Here we describe how scaffolds for tissue engineering can also be used as sensors for monitoring cellular activity such as adhesion and spreading. Carbon nanotube polymer composites were fabricated into membranes and scaffolds with electro-conductive properties. Impedance techniques were used to measure the effects of media and cell cultures on composite membranes and the results were analysed using lumped parameter models. We show that protein adhesion can be distinguished from cell adhesion as the impedance changes are much smaller for the latter (5%). In the presence of cells, impedance changes are of the order of 40% and can be correlated with adhesion, spreading and changes in cell density.

Original languageEnglish
Pages (from-to)3303-3308
Number of pages6
JournalBiosensors and Bioelectronics
Volume26
Issue number7
DOIs
Publication statusPublished - 15 Mar 2011

Keywords

  • Carbon nanotubes
  • Cells
  • Impedance monitoring
  • Poly-l-lactide
  • Scaffolds
  • Sensor

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