Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera

Agus Sjahrurachman; Beti Ernawati Dewi; R. Fera Ibrahim; Mardiastuti; Tjahjani Mirawati Sudiro; Pratiwi Pudjilestari Sudarmono

doi:10.13181/mji.v9i1.643

Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera

Agus Sjahrurachman, Beti Ernawati Dewi, R. Fera Ibrahim, Mardiastuti, Tjahjani Mirawati Sudiro, Pratiwi Pudjilestari Sudarmono

Research output: Contribution to journal › Article › peer-review

Abstract

Laboratory diagnosis of dengue infection plays an important role for case management and disease control. Unfortunately, many of the available laboratory tests are not appropriate in terms of rapidity and simplicity. Here, we report a new format of semiquantitative dot immunoassay for detection of IgM-anti dengue antibodies in human sera employing biotinylated-dengue antigens which is simple in term of methodology and rapid in term of the test result. The results indicated that the new test is sensitive for diagnosis of secondary dengue infection. The test result can be obtained within four and a half hours.

Original language	English
Pages (from-to)	9-12
Number of pages	4
Journal	Medical Journal of Indonesia
Volume	9
Issue number	1
DOIs	https://doi.org/10.13181/mji.v9i1.643
Publication status	Published - 1 Jan 2000

Keywords

Biotinylated-dengue
Diagnosis
Immunoassay

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abstract = "Laboratory diagnosis of dengue infection plays an important role for case management and disease control. Unfortunately, many of the available laboratory tests are not appropriate in terms of rapidity and simplicity. Here, we report a new format of semiquantitative dot immunoassay for detection of IgM-anti dengue antibodies in human sera employing biotinylated-dengue antigens which is simple in term of methodology and rapid in term of the test result. The results indicated that the new test is sensitive for diagnosis of secondary dengue infection. The test result can be obtained within four and a half hours.",

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AU - Sjahrurachman, Agus

AU - Dewi, Beti Ernawati

AU - Ibrahim, R. Fera

AU - Mardiastuti,

AU - Sudiro, Tjahjani Mirawati

AU - Sudarmono, Pratiwi Pudjilestari

PY - 2000/1/1

Y1 - 2000/1/1

N2 - Laboratory diagnosis of dengue infection plays an important role for case management and disease control. Unfortunately, many of the available laboratory tests are not appropriate in terms of rapidity and simplicity. Here, we report a new format of semiquantitative dot immunoassay for detection of IgM-anti dengue antibodies in human sera employing biotinylated-dengue antigens which is simple in term of methodology and rapid in term of the test result. The results indicated that the new test is sensitive for diagnosis of secondary dengue infection. The test result can be obtained within four and a half hours.

AB - Laboratory diagnosis of dengue infection plays an important role for case management and disease control. Unfortunately, many of the available laboratory tests are not appropriate in terms of rapidity and simplicity. Here, we report a new format of semiquantitative dot immunoassay for detection of IgM-anti dengue antibodies in human sera employing biotinylated-dengue antigens which is simple in term of methodology and rapid in term of the test result. The results indicated that the new test is sensitive for diagnosis of secondary dengue infection. The test result can be obtained within four and a half hours.

KW - Biotinylated-dengue

KW - Diagnosis

KW - Immunoassay

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EP - 12

JO - Medical Journal of Indonesia

JF - Medical Journal of Indonesia

IS - 1

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Semi-quantitative dot immunoassay for detection of IgM anti-dengue antibodies in human sera

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Keywords

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