TY - JOUR
T1 - Selective non-enzymatic uric acid sensing in the presence of dopamine
T2 - electropolymerized poly-pyrrole modified with a reduced graphene oxide/PEDOT:PSS composite
AU - Putra, Budi Riza
AU - Nisa, Ulfiatun
AU - Heryanto, Rudi
AU - Khalil, Munawar
AU - Khoerunnisa, Fitri
AU - Ridhova, Aga
AU - Thaha, Yudi Nugraha
AU - Marken, Frank
AU - Wahyuni, Wulan Tri
N1 - Funding Information:
We would like to thank the Ministry of Research and Technology, National Research and Innovation Agency Republic of Indonesia for the research funding (scheme PDUPT contract No. 3644/IT3.L1/PT.01.03/P/B/2022).
Publisher Copyright:
© 2022 The Royal Society of Chemistry.
PY - 2022/11/1
Y1 - 2022/11/1
N2 - A highly selective electrochemical sensor based on a molecularly imprinted polymer (MIP) to be developed for uric acid detection in the presence of dopamine as an interference molecule was demonstrated in this study. This non-enzymatic uric acid sensor was developed by electropolymerizing poly-pyrrole onto a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) on a glassy carbon electrode (GCE) to give MIP/ErGO/PEDOT:PSS electrodes. The structural properties, surface morphology, and electrochemical interface of this fabricated uric acid sensor was then characterized using infrared spectroscopy, scanning electron microscopy, atomic force microscopy, and electrochemical impedance spectroscopy. This fabricated non-enzymatic electrochemical sensor (MIP/ErGO/PEDOT:PSS modified GCE) also showed excellent analytical performance at the optimum ratio of monomer/template concentration and optimized electropolymerization conditions, for example in the low concentration range of 0.1-100 μM with a detection limit of 0.05 μM towards uric acid detection in the presence of dopamine. Furthermore, this fabricated uric acid sensor also exhibited good reproducibility and stability for uric acid measurements in the presence of dopamine for 5 consecutive days. In addition, this sensor demonstrates highly selective detection of uric acid in the presence of several interfering species such as dopamine, urea, glucose, magnesium ions, and ascorbic acid. This fabricated uric acid sensor ultimately showed satisfactory uric acid measurement in samples of human urine and is expected to be used in early-stage disease diagnosis.
AB - A highly selective electrochemical sensor based on a molecularly imprinted polymer (MIP) to be developed for uric acid detection in the presence of dopamine as an interference molecule was demonstrated in this study. This non-enzymatic uric acid sensor was developed by electropolymerizing poly-pyrrole onto a composite of electrochemically reduced graphene oxide (ErGO) and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS) on a glassy carbon electrode (GCE) to give MIP/ErGO/PEDOT:PSS electrodes. The structural properties, surface morphology, and electrochemical interface of this fabricated uric acid sensor was then characterized using infrared spectroscopy, scanning electron microscopy, atomic force microscopy, and electrochemical impedance spectroscopy. This fabricated non-enzymatic electrochemical sensor (MIP/ErGO/PEDOT:PSS modified GCE) also showed excellent analytical performance at the optimum ratio of monomer/template concentration and optimized electropolymerization conditions, for example in the low concentration range of 0.1-100 μM with a detection limit of 0.05 μM towards uric acid detection in the presence of dopamine. Furthermore, this fabricated uric acid sensor also exhibited good reproducibility and stability for uric acid measurements in the presence of dopamine for 5 consecutive days. In addition, this sensor demonstrates highly selective detection of uric acid in the presence of several interfering species such as dopamine, urea, glucose, magnesium ions, and ascorbic acid. This fabricated uric acid sensor ultimately showed satisfactory uric acid measurement in samples of human urine and is expected to be used in early-stage disease diagnosis.
UR - http://www.scopus.com/inward/record.url?scp=85141798776&partnerID=8YFLogxK
U2 - 10.1039/d2an01463g
DO - 10.1039/d2an01463g
M3 - Article
C2 - 36317598
AN - SCOPUS:85141798776
SN - 0003-2654
VL - 147
SP - 5334
EP - 5346
JO - Analyst
JF - Analyst
IS - 23
ER -
