TY - GEN
T1 - Selection of propolis Tetragonula sp. extract solvent with flavonoids and polyphenols concentration and antioxidant activity parameters
AU - Christina, Daisy
AU - Hermansyah, Heri
AU - Wijanarko, Anondho
AU - Rohmatin, Etin
AU - Sahlan, Muhamad
AU - Pratami, Diah Kartika
AU - Mun'im, Abdul
N1 - Publisher Copyright:
© 2018 Author(s). Published by AIP Publishing.
PY - 2018/2/13
Y1 - 2018/2/13
N2 - Antioxidants are inhibitory compounds that can inhibit auto oxidation reaction by binding to free radicals and highly reactive molecules. The human body needs antioxidant. Antioxidants can be obtained from a variety of natural ingredients, including propolis. Propolis is the natural sap of the bees, obtained from the herbs around the honeycomb. Ethanol is the solvent that often used to extract propolis. Although it has many advantages, ethanol also has weaknesses such as intolerance to alcohol by some people. Therefore, this research was to extract propolis Tetragonula sp. coarse (C) and soft (S) using four varieties of organic solvent, i.e. olive oil (OO), virgin coconut oil (VCO), propylene glycol (PG), and lecithin (L). It was expected to get the best solvent in extracting propolis. The selection of the best solvent was determined by total flavonoids and polyphenols content assay and antioxidant activity. At each test, the absorbance value read by a microplate reader. Flavonoids content assay is using AlCl3 method with best result on rough-VCO propolis extract of 2509,767 ± 615,02 μg/mL. Polyphenols content assay was using Folin Ciocalteu method with the best results on soft-VCO propolis extract of 1391 ± 171.47 μg/mL. Antioxidant activity assay is using DPPH method with best result on soft-VCO propolis extract with IC50 value of 1,559 ± 0,222 μg/mL.
AB - Antioxidants are inhibitory compounds that can inhibit auto oxidation reaction by binding to free radicals and highly reactive molecules. The human body needs antioxidant. Antioxidants can be obtained from a variety of natural ingredients, including propolis. Propolis is the natural sap of the bees, obtained from the herbs around the honeycomb. Ethanol is the solvent that often used to extract propolis. Although it has many advantages, ethanol also has weaknesses such as intolerance to alcohol by some people. Therefore, this research was to extract propolis Tetragonula sp. coarse (C) and soft (S) using four varieties of organic solvent, i.e. olive oil (OO), virgin coconut oil (VCO), propylene glycol (PG), and lecithin (L). It was expected to get the best solvent in extracting propolis. The selection of the best solvent was determined by total flavonoids and polyphenols content assay and antioxidant activity. At each test, the absorbance value read by a microplate reader. Flavonoids content assay is using AlCl3 method with best result on rough-VCO propolis extract of 2509,767 ± 615,02 μg/mL. Polyphenols content assay was using Folin Ciocalteu method with the best results on soft-VCO propolis extract of 1391 ± 171.47 μg/mL. Antioxidant activity assay is using DPPH method with best result on soft-VCO propolis extract with IC50 value of 1,559 ± 0,222 μg/mL.
KW - antioxidant activity
KW - flavonoid
KW - polyphenol
KW - propolis
KW - solvents
UR - http://www.scopus.com/inward/record.url?scp=85042375936&partnerID=8YFLogxK
U2 - 10.1063/1.5023967
DO - 10.1063/1.5023967
M3 - Conference contribution
AN - SCOPUS:85042375936
T3 - AIP Conference Proceedings
BT - 2nd Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Dhelika, Radon
A2 - Whulanza, Yudan
A2 - Ramahdita, Ghiska
A2 - Wulan, Praswasti P.D.K.
PB - American Institute of Physics Inc.
T2 - 2nd Biomedical Engineering's Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2017
Y2 - 25 July 2017 through 26 July 2017
ER -