Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells

Purnamawati; Jeanne Adiwinata; Andhika Rachman; Isabella Kurnia Liem; Septelia Inawati Wanandi

doi:10.1166/asl.2017.9376

Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells

Purnamawati, Jeanne Adiwinata, Andhika Rachman, Isabella Kurnia Liem, Septelia Inawati Wanandi

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

Introduction: Conditioned media of Mesenchymal Stem Cell’s culture containing secretomes has been identified to mediate communication between MSCs and cancer cells which could affect the stemness of cancer stem cells. Methods: Conditioned media (CM) of Adipose Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs) was collected and supplemented to Breast Cancer Stem Cells (BCSCs) ALDH+ cells for 72 hours. The expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs was determined using qRT-PCR technique, while the viability was measured using Tryphan blue exclusion assay. Results: CM of ASCs (50% v/v) could significantly increase both cell viability and the expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs. In contrast, CM of UCSCs (50% v/v) could significantly decrease the expression of ALDH1A1 gene and decrease the viability of BCSCs without affecting Oct4 and Sox2 expression. Conclusion: ALDH1A1 was differentially expressed in BCSCs after supplementation with secretomes of ASCs and UCSCs. Further studies are required to confirm the role of MSCs secretomes on the pluripotency and viability of BCSCs.

Original language	English
Pages (from-to)	6701-6704
Number of pages	4
Journal	Advanced Science Letters
Volume	23
Issue number	7
DOIs	https://doi.org/10.1166/asl.2017.9376
Publication status	Published - 1 Jul 2017

Keywords

ALDH1A1
ASCs
BCSCs
Secretomes
UCSCs

Access to Document

10.1166/asl.2017.9376

Fingerprint Dive into the research topics of 'Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells'. Together they form a unique fingerprint.

Cite this

@article{faa927e342314f7d85f61d5dc5d92abc,

title = "Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells",

abstract = "Introduction: Conditioned media of Mesenchymal Stem Cell{\textquoteright}s culture containing secretomes has been identified to mediate communication between MSCs and cancer cells which could affect the stemness of cancer stem cells. Methods: Conditioned media (CM) of Adipose Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs) was collected and supplemented to Breast Cancer Stem Cells (BCSCs) ALDH+ cells for 72 hours. The expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs was determined using qRT-PCR technique, while the viability was measured using Tryphan blue exclusion assay. Results: CM of ASCs (50% v/v) could significantly increase both cell viability and the expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs. In contrast, CM of UCSCs (50% v/v) could significantly decrease the expression of ALDH1A1 gene and decrease the viability of BCSCs without affecting Oct4 and Sox2 expression. Conclusion: ALDH1A1 was differentially expressed in BCSCs after supplementation with secretomes of ASCs and UCSCs. Further studies are required to confirm the role of MSCs secretomes on the pluripotency and viability of BCSCs.",

keywords = "ALDH1A1, ASCs, BCSCs, Secretomes, UCSCs",

author = "Purnamawati and Jeanne Adiwinata and Andhika Rachman and Liem, {Isabella Kurnia} and Wanandi, {Septelia Inawati}",

year = "2017",

month = jul,

day = "1",

doi = "10.1166/asl.2017.9376",

language = "English",

volume = "23",

pages = "6701--6704",

journal = "Advanced Science Letters",

issn = "1936-6612",

publisher = "American Scientific Publishers",

number = "7",

}

TY - JOUR

T1 - Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells

AU - Purnamawati,

AU - Adiwinata, Jeanne

AU - Rachman, Andhika

AU - Liem, Isabella Kurnia

AU - Wanandi, Septelia Inawati

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Introduction: Conditioned media of Mesenchymal Stem Cell’s culture containing secretomes has been identified to mediate communication between MSCs and cancer cells which could affect the stemness of cancer stem cells. Methods: Conditioned media (CM) of Adipose Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs) was collected and supplemented to Breast Cancer Stem Cells (BCSCs) ALDH+ cells for 72 hours. The expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs was determined using qRT-PCR technique, while the viability was measured using Tryphan blue exclusion assay. Results: CM of ASCs (50% v/v) could significantly increase both cell viability and the expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs. In contrast, CM of UCSCs (50% v/v) could significantly decrease the expression of ALDH1A1 gene and decrease the viability of BCSCs without affecting Oct4 and Sox2 expression. Conclusion: ALDH1A1 was differentially expressed in BCSCs after supplementation with secretomes of ASCs and UCSCs. Further studies are required to confirm the role of MSCs secretomes on the pluripotency and viability of BCSCs.

AB - Introduction: Conditioned media of Mesenchymal Stem Cell’s culture containing secretomes has been identified to mediate communication between MSCs and cancer cells which could affect the stemness of cancer stem cells. Methods: Conditioned media (CM) of Adipose Stem Cells (ASCs) and Umbilical Cord Stem Cells (UCSCs) was collected and supplemented to Breast Cancer Stem Cells (BCSCs) ALDH+ cells for 72 hours. The expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs was determined using qRT-PCR technique, while the viability was measured using Tryphan blue exclusion assay. Results: CM of ASCs (50% v/v) could significantly increase both cell viability and the expression of ALDH1A1, Oct4 and Sox2 genes in BCSCs. In contrast, CM of UCSCs (50% v/v) could significantly decrease the expression of ALDH1A1 gene and decrease the viability of BCSCs without affecting Oct4 and Sox2 expression. Conclusion: ALDH1A1 was differentially expressed in BCSCs after supplementation with secretomes of ASCs and UCSCs. Further studies are required to confirm the role of MSCs secretomes on the pluripotency and viability of BCSCs.

KW - ALDH1A1

KW - ASCs

KW - BCSCs

KW - Secretomes

KW - UCSCs

UR - http://www.scopus.com/inward/record.url?scp=85030233495&partnerID=8YFLogxK

U2 - 10.1166/asl.2017.9376

DO - 10.1166/asl.2017.9376

M3 - Article

AN - SCOPUS:85030233495

VL - 23

SP - 6701

EP - 6704

JO - Advanced Science Letters

JF - Advanced Science Letters

SN - 1936-6612

IS - 7

ER -

Secretomes of adipose and umbilical cord-derived stem cells affect ALDH1A1 expression in breast cancer stem cells

Abstract

Keywords

Access to Document

Other files and links

Cite this