TY - GEN
T1 - Secretome analysis of dental pulp stem cell (DPSC) for hard tissue regeneration
AU - Bachtiar, Endang Winiati
AU - Ali, Soegianto
AU - Vitria, Evy Eida
AU - Sugianto, Tjong David
N1 - Publisher Copyright:
© 2024 AIP Publishing LLC.
PY - 2024/2/6
Y1 - 2024/2/6
N2 - Secretome is a breakthrough for modern medicine application due to its high availability and capability to induce tissue regeneration. Research regarding cartilage recovery is vital because cartilage tissue can be found in multiple essential locations in the body but lacks regenerative properties. This research aims to identify the profile of proteins inside the secretome secreted from a dental pulp stem cell (DPSC) using HPLC and indirect ELISA, specifically, the BMP-2 protein. The mobile phase used for HPLC analysis was 2% acetonitrile solution for 30 minutes. For the indirect ELISA method, we incubated the samples overnight in a cold environment. The antibodies bound to the samples were incubated for 2 and 1 hours with a washing step after each incubation. The color was developed using TMB substrate and then analyzed in 450 nm wavelength. Our results showed that the dynamic of BMP-2 concentration follows a similar pattern to the pattern in animal cell growth. Conversely, BMP-2 concentration increased exponentially during the log phase of DPSC (6-7 days). Thus, we concluded that DPSC naturally produces high levels of BMP-2, and its concentration peaks at 6-7 days of culture. We posit that the secretome of DPSC has a promising potential as the source of signaling molecules for hard tissue engineering.
AB - Secretome is a breakthrough for modern medicine application due to its high availability and capability to induce tissue regeneration. Research regarding cartilage recovery is vital because cartilage tissue can be found in multiple essential locations in the body but lacks regenerative properties. This research aims to identify the profile of proteins inside the secretome secreted from a dental pulp stem cell (DPSC) using HPLC and indirect ELISA, specifically, the BMP-2 protein. The mobile phase used for HPLC analysis was 2% acetonitrile solution for 30 minutes. For the indirect ELISA method, we incubated the samples overnight in a cold environment. The antibodies bound to the samples were incubated for 2 and 1 hours with a washing step after each incubation. The color was developed using TMB substrate and then analyzed in 450 nm wavelength. Our results showed that the dynamic of BMP-2 concentration follows a similar pattern to the pattern in animal cell growth. Conversely, BMP-2 concentration increased exponentially during the log phase of DPSC (6-7 days). Thus, we concluded that DPSC naturally produces high levels of BMP-2, and its concentration peaks at 6-7 days of culture. We posit that the secretome of DPSC has a promising potential as the source of signaling molecules for hard tissue engineering.
KW - bone morphogenic protein (BMP)
KW - Conditioned-medium
KW - dental pulp stem cell
KW - Secretome
KW - stem cells
KW - tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85185805473&partnerID=8YFLogxK
U2 - 10.1063/5.0144052
DO - 10.1063/5.0144052
M3 - Conference contribution
AN - SCOPUS:85185805473
T3 - AIP Conference Proceedings
BT - AIP Conference Proceedings
A2 - Kusuma, Andyka
A2 - Fatriansyah, Jaka Fajar
A2 - Dhelika, Radon
A2 - Pratama, Mochamad Adhiraga
A2 - Irwansyah, Ridho
A2 - Maknun, Imam Jauhari
A2 - Putra, Wahyuaji Narottama
A2 - Ardi, Romadhani
A2 - Harwahyu, Ruki
A2 - Harahap, Yulia Nurliani
A2 - Lischer, Kenny
PB - American Institute of Physics Inc.
T2 - 17th International Conference on Quality in Research, QiR 2021 in conjunction with the International Tropical Renewable Energy Conference 2021, I-Trec 2021 and the 2nd AUN-SCUD International Conference, CAIC-SIUD
Y2 - 13 October 2021 through 15 October 2021
ER -