Secang heartwood extract in serial dilution as antibacterial agent against Biofilm E. faecalis clinical isolate

E. faecalis bacteria are frequently encountered in persistent and resistant root canal infections. Their ability to form biofilms contributes to its persistence. Importantly, chemical-based irrigation usage is linked to side effects. Caesalpinia Sappan Linn, known as Secang heartwood, has been traditionally used for a variety of medical purposes, including antimicrobial. We aimed analyze the antibacterial effects of Secang heartwood extract solution on E. faecalis biofilms. E. faecalis biofilms from clinical isolates were grown on microtiter well plates, incubated for 24 h and subjected to various concentrations of Secang heartwood extract solution: 312 μg/ml, 625 μg/ml, 1250 μg/ml, 2500 μg/ml, 5000 μg/ml (and CHX 2% as control). Following a 15 min incubation, wells were rinsed with PBS and scrapped off. Biofilms were diluted, spread on a solid medium and incubated for 24 h. Subsequently, viable grown colonies were counted. The concentration of Secang heartwood extract solution that produced the least inhibition of E. faecalis bacteria growth was 5000 μg/ml. At a concentration as low as 625 μg/ml, we observed no colony growth. Secang heartwood extract solution has an antibacterial effect on biofilm of E. faecalis bacteria. The concentration of aqueous extract of 625 μg/ml has an antibacterial power equivalent to CHX 2%, representing the optimal antibacterial concentration.