TY - JOUR
T1 - Screening for amylolytic activity and characterization of thermophilic Actinobacteria isolated from a geothermal area in West Java, Indonesia
AU - Syafitri, Winda Ayu
AU - Ningsih, Fitria
AU - Setyaningsih, Putri Pratiwi
AU - Rachmania, Mazytha Kinanti
AU - Sari, Dhian Chitra Ayu Fitria
AU - Yabe, Shuhei
AU - Yokota, Akira
AU - Oetari, Ariyanti
AU - Sjamsuridzal, Wellyzar
N1 - Funding Information:
This research was funded by Hibah Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa (PITTA) UI 2018 no. 2331/UN2.R3.1/HKP.05.00/2018 to W.S. We would like to acknowledge the financial assistance provided by the Department of Microbial Resources, Graduate School of Agricultural Science, Faculty of Agriculture, Tohoku University, Japan.
Publisher Copyright:
© 2019, Society for Indonesian Biodiversity. All rights reserved.
PY - 2019/7/1
Y1 - 2019/7/1
N2 - In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45°C. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60°C. Fifteen of the 17 isolates showed amylolytic activity at 45°C, 13 showed such activity at 50°C, and four showed activity at 55°C. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60°C. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.
AB - In this study, we describe the screening for amylolytic activity of 17 thermophilic Actinobacteria isolates obtained from the soil of Cisolok geysers, a geothermal area in West Java, Indonesia. All isolates were screened for amylolytic activity by the starch-agar plate method at various temperatures. The results showed that all of isolates were able to grow at 45°C. The growth abilities of the isolates grown in ISP 1 medium varied at temperatures from 45 to 60°C. Fifteen of the 17 isolates showed amylolytic activity at 45°C, 13 showed such activity at 50°C, and four showed activity at 55°C. Only three isolates, designated SL1-2-R-2, SL1-2-R-3, and SL1-2-R-4, showed growth and amylolytic activity at 60°C. These three isolates were selected for molecular identification. The nearly full-length of 16S rRNA gene sequences data showed that these three isolates have a similarity of 99.93-100% with Actinomadura keratinilytica WCC-2265T and of 98.74-98.91% with A. miaoliensis BC 44T-5T. Phylogenetic tree shows that all three isolates are clustered together in a monophyletic group with the type strain of A. keratinilytica WCC-2265T as their most closely related species, with 100% bootstrap support. Based on sequencing of the 16S rRNA gene, phylogenetic comparison, and phenotypic characterization, the three isolates were identified as A. keratinilytica.
KW - Amylolytic activity
KW - Geothermal area
KW - Soil
KW - Thermophilic actinobacteria
UR - http://www.scopus.com/inward/record.url?scp=85070809316&partnerID=8YFLogxK
U2 - 10.13057/biodiv/d200720
DO - 10.13057/biodiv/d200720
M3 - Article
AN - SCOPUS:85070809316
VL - 20
SP - 1929
EP - 1938
JO - Biodiversitas
JF - Biodiversitas
SN - 1412-033X
IS - 7
ER -