TY - JOUR
T1 - Safety evaluation of human peripheral blood mononuclear cells in naive rats:a chronic toxicitystudy
AU - Supartono, Basuki
AU - Farida, Siti
AU - Suhandono, Sony
AU - Yusuf, Ahmad Aulia
N1 - Funding Information:
We thank UPN Veteran Jakarta for a research grant (No: 22/UN.61.4/LIT/2019), Stem Cell Laboratory of UPN Veteran Jakarta for technical support, Al Fauzan General Hospital Jakarta, Indonesia for providing donor, and ISTN Jakarta for providing
Publisher Copyright:
© 2022, Ibn Sina Trust. All rights reserved.
PY - 2022/2/25
Y1 - 2022/2/25
N2 - Human peripheral blood mononuclear cells (PBMC) are widely used within tissue engineering therapy, thus, the evaluation of their safety is mandatory. The present study aims to assess the safety of human PBMC transplantations in naive rats in terms of chronic toxicity. Rats received intravenous injections of human PBMC with doses of 105, 106, or 107 cells (n = 5/group/sex) every month for three months without administration of any immunosuppressant drugs. We evaluated clinical, physical, laboratory, and pathology parameters. No morbidity, mortality, or significant differences occurred within all of the tested parameters between control and experimental groups until the finalization of the study. In conclusion, the current work indicates that human PBMC transplantation in naive rats did not induce chronic toxicity reaction.
AB - Human peripheral blood mononuclear cells (PBMC) are widely used within tissue engineering therapy, thus, the evaluation of their safety is mandatory. The present study aims to assess the safety of human PBMC transplantations in naive rats in terms of chronic toxicity. Rats received intravenous injections of human PBMC with doses of 105, 106, or 107 cells (n = 5/group/sex) every month for three months without administration of any immunosuppressant drugs. We evaluated clinical, physical, laboratory, and pathology parameters. No morbidity, mortality, or significant differences occurred within all of the tested parameters between control and experimental groups until the finalization of the study. In conclusion, the current work indicates that human PBMC transplantation in naive rats did not induce chronic toxicity reaction.
KW - Chronic toxicity
KW - Human peripheral blood mononuclear cells
KW - Naive rat
KW - Stemcell transplantation
KW - Tissue engineering
UR - http://www.scopus.com/inward/record.url?scp=85125619475&partnerID=8YFLogxK
U2 - 10.3329/bjms.v21i2.57029
DO - 10.3329/bjms.v21i2.57029
M3 - Article
AN - SCOPUS:85125619475
SN - 2223-4721
VL - 21
SP - 373
EP - 383
JO - Bangladesh Journal of Medical Science
JF - Bangladesh Journal of Medical Science
IS - 2
ER -
