RT-PCR (REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION): SUATU CARA PENDETEKSI PERUBAHAN-PERUBAHAN EKSPRESI GEN PADA PENYAKIT

The reverse transcription polymerase chain reaction (RT-PCR) is widely applied for detecting mRNA expression and cloning cDNA. This method consists of two parts: cDNA synthesis from mRNA by reverse transcription (RT) and amplification of cDNA by PCR. The RT-PCR has proved useful in amplifying very small amount of specific mRNA to obtain cDNA clones and to detect mRNA transcripts from abnormal cells in a background of normal cells.