BACKGROUND: Tuberculosis (TB) remains to be a leading cause of morbidity and mortality worldwide. The immune defense against Mycobacterium tuberculosis is complicated. Interferon-gamma (IFN-γ) is the main cytokine involved in the immune response of TB. To date, the role of +874 A/T single-nucleotide polymorphism (SNP) and TB disease susceptibility continues to be controversial. AIM: The aim of this study was to investigate the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia. METHODS: A case–control study was conducted in Medan and Batubara, North Sumatera, Indonesia, from January to December 2016. A total of 51 children with TB and 51 healthy controls were enrolled in this study. Subjects were 2 months–14 years old age children diagnosed with TB and written informed consent from the parents or the caregivers to participate. Subjects were withdrawn from the study when immunodeficiency condition was found or suffered from other infection disease. DNA samples were obtained from all of the subjects. +874 A/T SNP was identified by performing the amplification refractory mutational system-polymerase chain reaction method. IFN-γ levels were measured using human enzyme-linked immunosorbent assay. Data analysis was performed using Chi-square and Mann–Whitney test. P < 0.05 was considered statistically significant. RESULTS: The result of this study reveals that the presence of AA, AT, and TT genotype in TB patients was 31 (60.8%), 20 (39.2%), and 0 (0%), respectively (p = 0.023). Significant decreased production of IFN-γ levels (p = 0.042) was found in TB patients 9.41 (1.10–28.06) pg/ml. CONCLUSION: Our study demonstrated significant evidence of the role of +874 A/T SNP and TB disease susceptibility of pediatric population in North Sumatera, Indonesia, predominantly AA genotype. Significant decreased production of IFN-γ reported among pediatric TB.
|Number of pages||4|
|Journal||Open Access Macedonian Journal of Medical Sciences|
|Publication status||Published - 1 Oct 2021|
- +874 A/T
- Single-nucleotide polymorphism