Role of glycodeoxycholic acid to induce acute pancreatitis in Macaca nemestrina

Achmad Fauzi, Suhendro, Marcellus Simadibrata, Abdul Azis Rani, Dondin Sajuthi, Permanawati, Rosvitha Amanda, Dadang Makmun

Research output: Contribution to journalArticlepeer-review

Abstract

Background: Acute pancreatitis exhibits a rapid clinical progression which makes it difficult to observe in human; hence, an experimental animal model is needed. This preliminary study performed an induction of acute pancreatitis using glycodeoxycholic acid (GDOC) in an experimental macaque model. Methods: GDOC injections (initial dose of 11.20 mg/kg) were administered in an escalating manner at specific time points. The injection was given along the bilio-pancreatic duct, followed by measurement of vital signs, serum amylase-lipase, TNF-α, procalcitonin, oxidative stress parameters, and microscopic and macroscopic findings. Results: The results indicated that acute pancreatitis occurred following induction with low-dose GDOC. Serum amylase and lipase levels increased with subsequent GDOC injections. Blood pressure and heart rate were elevated, indicating abdominal pain. Changes in TNF-α, procalcitonin, and oxidative stress values showed active inflammation. We observed histologic features of pancreatitis and as the dose increased, vasodilation of the splanchnic vasculatures was observed. Conclusions: Small dose GDOC injection in the bilio-pancreatic duct may have a role to induce acute pancreatitis in Macaca nemestrina.

Original languageEnglish
Pages (from-to)134-142
Number of pages9
JournalJournal of Medical Primatology
Volume51
Issue number3
DOIs
Publication statusPublished - 20 Mar 2022

Keywords

  • acute pancreatitis
  • experimental
  • glycodeoxycholic acid
  • Macaca nemestrina

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