Rodent is potential reservoir of zoonoses fungi in Jakarta, Indonesia

Sem Samuel Surja, Robiatul Adawiyah, Anna Rozaliyani, Ridhawati Sjam, Retno Wahyuningsih

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Some potentially human pathogenic fungi are known to be zoonoses. Rodent is one of potential reservoir of fungi. To date, little is known about the role of rodent in transmitting fungal disease in Indonesia. Therefore, purpose of this study was to find evidence of potential fungal zoonoses in rodent. We caught two house rats, one rat was from the house of patient suffering from talaromycosis and the other one was from a healthy person house. These rats internal organs (lung, liver, and spleen) were inoculated onto sabouraud dextrose agar (SDA) and SDA with additional of chloramphenicol. Fungi grown in the medium were analyzed using polymerase chain reaction (PCR) of internal transcribed spacer (ITS) continued by sequence-based approach. In addition PCR was also conducted using primers developed from beta tubulin gene. Amplified regions were sequenced and compared to database that contains reference sequences. We found three fungal species. Talaromyces atroroseus was isolated from the rat that caught from the house of patient with talaromycosis, while Purpureocillium lilacinum and Penicillium citrinum were isolated from the other rat caught in the house of healthy individual. Although naturally could be found in the environment, these species had been reported to cause fatal systemic mycosis in human. In conclusion Talaromyces atroroseus, Purpureocillium lilacinum and P. citrinum could be found in rat. This result indicates that rat could be a reservoir for these fungi.

Original languageEnglish
Title of host publicationInternational Conference on Bioinformatics and Nanomedicine from Natural Resources for Biomedical Research
Subtitle of host publication3rd Annual Scientific Meeting for Biomedical Sciences
EditorsWindy Yuliana Budianto, Hidayat Sujuti, Husnul Khotimah
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418400
DOIs
Publication statusPublished - 4 Jun 2019
Event3rd Annual Scientific Meeting of Indonesian Consortium for Biomedical Sciences, ASMICBS 2018 - Malang, Indonesia
Duration: 21 Nov 201823 Nov 2018

Publication series

NameAIP Conference Proceedings
Volume2108
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd Annual Scientific Meeting of Indonesian Consortium for Biomedical Sciences, ASMICBS 2018
CountryIndonesia
CityMalang
Period21/11/1823/11/18

Keywords

  • fungi
  • Jakarta
  • mycosis
  • rat
  • zoonoses

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