TY - JOUR
T1 - Risk factors for early childhood caries based on identification of Veillonella spp. Using RT-PCR
AU - Setiawan, Asty Samiaty
AU - Darwita, Risqa Rina
AU - Susilawati, Sri
AU - Maharani, Diah Ayu
AU - Djais, Ariadna Adisattya
N1 - Funding Information:
Directorate Research and Community Engagement Universitas of Indonesia (Grant No. NKB-0134/UN2.R3.1/HKP.05.00/2019).
Publisher Copyright:
© 2020, Association of Support to Oral Health Research (APESB). All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2–3 years. Material and Methods: A cross-sectional survey was conducted and samples from 87 children aged 2–3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test. Results: A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83. Conclusion: The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
AB - Objective: To identify the occurrence of Veillonella spp. in children using real-time PCR (RT-PCR) and to determine its role as a risk factor for ECC in children aged 2–3 years. Material and Methods: A cross-sectional survey was conducted and samples from 87 children aged 2–3 years, who lived in selected villages in the Bandung City area, Indonesia, were collected. Examination for dental caries was performed using standard checks for decay, missing, and filled surfaces (dfms), and saliva samples were taken. Microbiological examination was performed using RT-PCR with primers consisting of one primary set for Veillonella spp. and one universal primary set for 16S rDNA. We performed statistical testing using the Mann Whitney rank-sum test. Results: A total of 87 children were sampled, and an ECC prevalence of 71.3% was found, with a mean dmfs of 7.1 (± 9.1). The proportion of Veillonella spp. in caries-free children was 2.13 ± 2.30, while in children with ECC, it was 3.29 ± 6.83. Conclusion: The proportion of Veillonella spp. in children with ECC was higher than in caries-free children; therefore, Veillonella spp. may be a risk factor for ECC.
KW - Dental caries
KW - Gram-negative bacteria
KW - Polymerase chain reaction
UR - http://www.scopus.com/inward/record.url?scp=85090693267&partnerID=8YFLogxK
U2 - 10.1590/pboci.2020.147
DO - 10.1590/pboci.2020.147
M3 - Article
AN - SCOPUS:85090693267
SN - 1519-0501
VL - 20
SP - 1
EP - 8
JO - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
JF - Pesquisa Brasileira em Odontopediatria e Clinica Integrada
M1 - e5701
ER -