RETINOL STABILITY IN ANTI-AGING FACE SERUM POST-OPENING PERIOD

Baitha Palanggatan Maggadani, Risa Rahmayati, Taufiq Indra Rukmana, Callista Andinie Mulyadi

Research output: Contribution to journalArticlepeer-review

Abstract

Objective: This study aimed to evaluate the stability of retinol in facial serum products under various storage conditions during a specific period after the product is opened. Retinol analysis will be conducted using reversed-phase high-performance liquid chromatography with a UV detector. Methods: The optimum analytical method in this study was validated according to the ICH Q2 (R1) guideline. The chromatographic separation of retinol was achieved on RP-HPLC isocratic elution method at a wavelength of 324 nm, C18 column, mobile phase of methanol-acetonitrile (90:10), and a flow rate of 0.8 ml/min. Results: The recovery value for this method was 98.06%-101.6,6%, with RSD ≤ 2%. Limit of Detection (LOD) and limit of Quantification (LOQ) values obtained were 1.1819 µg/ml and 3.9399 µg/ml. Determining retinol levels in face serum samples with variation in storage conditions was conducted on d 0, 7, 14, 21, and 30. The retinol levels in all storage conditions decreased over the 30 d, ranging from 25% to 79%. The highest decrease was observed in samples stored in transparent containers and exposed to light, with a reduction of 79% and an average weekly decrease of 17%. Conclusion: Stability evaluation of retinol in the serum formulation showed decreased retinol concentration over time during storage. Transferring the product to a secondary container and exposure to light resulted in a higher concentration decrease than storing it in the original bottle.

Original languageEnglish
Pages (from-to)108-113
Number of pages6
JournalInternational Journal of Applied Pharmaceutics
Volume16
Issue number4
DOIs
Publication statusPublished - 1 Jul 2024

Keywords

  • Face serum
  • HPLC
  • Retinol
  • Stability

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