TY - JOUR
T1 - RETINOL STABILITY IN ANTI-AGING FACE SERUM POST-OPENING PERIOD
AU - Maggadani, Baitha Palanggatan
AU - Rahmayati, Risa
AU - Rukmana, Taufiq Indra
AU - Mulyadi, Callista Andinie
N1 - Publisher Copyright:
© 2024 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
PY - 2024/7/1
Y1 - 2024/7/1
N2 - Objective: This study aimed to evaluate the stability of retinol in facial serum products under various storage conditions during a specific period after the product is opened. Retinol analysis will be conducted using reversed-phase high-performance liquid chromatography with a UV detector. Methods: The optimum analytical method in this study was validated according to the ICH Q2 (R1) guideline. The chromatographic separation of retinol was achieved on RP-HPLC isocratic elution method at a wavelength of 324 nm, C18 column, mobile phase of methanol-acetonitrile (90:10), and a flow rate of 0.8 ml/min. Results: The recovery value for this method was 98.06%-101.6,6%, with RSD ≤ 2%. Limit of Detection (LOD) and limit of Quantification (LOQ) values obtained were 1.1819 µg/ml and 3.9399 µg/ml. Determining retinol levels in face serum samples with variation in storage conditions was conducted on d 0, 7, 14, 21, and 30. The retinol levels in all storage conditions decreased over the 30 d, ranging from 25% to 79%. The highest decrease was observed in samples stored in transparent containers and exposed to light, with a reduction of 79% and an average weekly decrease of 17%. Conclusion: Stability evaluation of retinol in the serum formulation showed decreased retinol concentration over time during storage. Transferring the product to a secondary container and exposure to light resulted in a higher concentration decrease than storing it in the original bottle.
AB - Objective: This study aimed to evaluate the stability of retinol in facial serum products under various storage conditions during a specific period after the product is opened. Retinol analysis will be conducted using reversed-phase high-performance liquid chromatography with a UV detector. Methods: The optimum analytical method in this study was validated according to the ICH Q2 (R1) guideline. The chromatographic separation of retinol was achieved on RP-HPLC isocratic elution method at a wavelength of 324 nm, C18 column, mobile phase of methanol-acetonitrile (90:10), and a flow rate of 0.8 ml/min. Results: The recovery value for this method was 98.06%-101.6,6%, with RSD ≤ 2%. Limit of Detection (LOD) and limit of Quantification (LOQ) values obtained were 1.1819 µg/ml and 3.9399 µg/ml. Determining retinol levels in face serum samples with variation in storage conditions was conducted on d 0, 7, 14, 21, and 30. The retinol levels in all storage conditions decreased over the 30 d, ranging from 25% to 79%. The highest decrease was observed in samples stored in transparent containers and exposed to light, with a reduction of 79% and an average weekly decrease of 17%. Conclusion: Stability evaluation of retinol in the serum formulation showed decreased retinol concentration over time during storage. Transferring the product to a secondary container and exposure to light resulted in a higher concentration decrease than storing it in the original bottle.
KW - Face serum
KW - HPLC
KW - Retinol
KW - Stability
UR - http://www.scopus.com/inward/record.url?scp=85199061057&partnerID=8YFLogxK
U2 - 10.22159/ijap.2024v16i4.50903
DO - 10.22159/ijap.2024v16i4.50903
M3 - Article
AN - SCOPUS:85199061057
SN - 0975-7058
VL - 16
SP - 108
EP - 113
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - 4
ER -