TY - JOUR
T1 - Relative expression of aldehyde dehydrogenase 1 family member A1 in different malignancies of human glioma cells
AU - Tango, T.
AU - Ichwan, S.
AU - Wanandi, S. I.
AU - Hardiany, N. S.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell (CSC) marker that plays a role in the differentiation and metastasis of human glioma cells. In this study, we aimed to analyze the expression of ALDH1A1 in different grades of malignancy of human glioma cells. Samples were collected from 32 patients at Cipto Mangunkusumo Hospital, resulting in 19 low-grade glioma (LGG) specimens, 11 high-grade glioma (HGG) specimens, and 2 normal brain specimens. Isolation of RNA was performed prior to the measurement of the relative quantification of ALDH1A1 by using the quantitative reverse transcription polymerase chain reaction (RT-qPCR). There was a trend of higher overexpression of ALDH1A1 in HGG compared to LGG in humans. However, this finding did not reach the level of statistical significance. ALDH1A1 may be a potential marker for malignancy classification. Therefore, further research is needed.
AB - Aldehyde dehydrogenase 1A1 (ALDH1A1) is a cancer stem cell (CSC) marker that plays a role in the differentiation and metastasis of human glioma cells. In this study, we aimed to analyze the expression of ALDH1A1 in different grades of malignancy of human glioma cells. Samples were collected from 32 patients at Cipto Mangunkusumo Hospital, resulting in 19 low-grade glioma (LGG) specimens, 11 high-grade glioma (HGG) specimens, and 2 normal brain specimens. Isolation of RNA was performed prior to the measurement of the relative quantification of ALDH1A1 by using the quantitative reverse transcription polymerase chain reaction (RT-qPCR). There was a trend of higher overexpression of ALDH1A1 in HGG compared to LGG in humans. However, this finding did not reach the level of statistical significance. ALDH1A1 may be a potential marker for malignancy classification. Therefore, further research is needed.
UR - http://www.scopus.com/inward/record.url?scp=85054509767&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032059
DO - 10.1088/1742-6596/1073/3/032059
M3 - Conference article
AN - SCOPUS:85054509767
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032059
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -