TY - JOUR
T1 - Relationships between Solobacterium moorei and Prevotella intermedia in subgingival microbiota of periodontitis patients with halitosis
T2 - A preliminary study using qPCR
AU - Bachtiar, Boy Muchlis
AU - Soeroso, Yuniarti
AU - Sunarto, Hari
AU - Maitimu, Fergy Christin
AU - Bachtiar, Endang Winiati
N1 - Funding Information:
This study was supported by Universitas Indonesia, through the PUTI Q2 Grant, No: BA-629/UN2.RST/PPM.00.03.01/2021. The authors acknowledge Asti, Vivi, and Anissa for laboratory assistance works. We would like to thank Editage (www.editage.com) for English language editing.
Publisher Copyright:
© 2022
PY - 2022/2/12
Y1 - 2022/2/12
N2 - Objective: Solobacterium moorei is suggested to be associated with the production of volatile sulphur compounds (VSCs) and can be found in subgingival plaques of deep periodontal pockets. We examined whether this bacterium's count was reduced in periodontitis patients with halitosis following non-surgical periodontal treatment, while the bacterial count of Prevotella intermedia was measured simultaneously as a control. Material & methods: This clinical study included 20 adults with chronic periodontitis who complained of halitosis. The bacterial relationship in the subgingival plaque sample was measured after 8 weeks post-treatment, including the probing pocket depth (PPD). Quantitative real-time PCR (qPCR) was used to measure the proportion of S. moorei, while the concentrations of H2S and CH3SH were determined using oral ChromaTM. Results: The presence of S. moorei was consistently observed in participants with periodontitis before and after non-surgical periodontal treatment and consistent showed a significantly lower proportion compared with P. intermedia. Solobacterium moorei showed a strong positive correlation with H2S and CH3SH concentrations, but a negative correlation with deep periodontal pocket measurements. Conversely, reduced P. intermedia may be more associated with a deep pocket, independent of the concentration of CH3SH. Conclusion: The study data showed that the proportion of S. moorei in the subgingival biofilm can be related to halitosis in periodontitis patients.
AB - Objective: Solobacterium moorei is suggested to be associated with the production of volatile sulphur compounds (VSCs) and can be found in subgingival plaques of deep periodontal pockets. We examined whether this bacterium's count was reduced in periodontitis patients with halitosis following non-surgical periodontal treatment, while the bacterial count of Prevotella intermedia was measured simultaneously as a control. Material & methods: This clinical study included 20 adults with chronic periodontitis who complained of halitosis. The bacterial relationship in the subgingival plaque sample was measured after 8 weeks post-treatment, including the probing pocket depth (PPD). Quantitative real-time PCR (qPCR) was used to measure the proportion of S. moorei, while the concentrations of H2S and CH3SH were determined using oral ChromaTM. Results: The presence of S. moorei was consistently observed in participants with periodontitis before and after non-surgical periodontal treatment and consistent showed a significantly lower proportion compared with P. intermedia. Solobacterium moorei showed a strong positive correlation with H2S and CH3SH concentrations, but a negative correlation with deep periodontal pocket measurements. Conversely, reduced P. intermedia may be more associated with a deep pocket, independent of the concentration of CH3SH. Conclusion: The study data showed that the proportion of S. moorei in the subgingival biofilm can be related to halitosis in periodontitis patients.
KW - CHSH
KW - HS
KW - Halitosis
KW - P. intermedia
KW - Periodontitis
KW - S. moorei
UR - http://www.scopus.com/inward/record.url?scp=85124886217&partnerID=8YFLogxK
U2 - 10.1016/j.sdentj.2022.02.003
DO - 10.1016/j.sdentj.2022.02.003
M3 - Article
AN - SCOPUS:85124886217
SN - 1013-9052
VL - 34
SP - 211
EP - 219
JO - Saudi Dental Journal
JF - Saudi Dental Journal
IS - 3
ER -