Single-nucleotide polymorphism (SNP) in the tumor necrosis factor alpha (TNF-α) gene occurring in the promoter region has been associated with several inflammatory diseases. Periodontitis, an inflammatory disease in tooth-supporting tissue that has a complex multifactorial etiology, affects males more often than females. This study aimed to discover the distribution of TNF-α G-308A polymorphism and its association with periodontitis susceptibility in Indonesian males. One hundred stored samples of biological DNA extracted from 50 periodontitis patients and 50 healthy controls were analyzed for TNF-α G-308A polymorphism by polymerase chain reaction– restriction fragment length polymorphism. The PCR product was digested with NcoI restriction enzyme. The AA genotype was absent in both periodontitis patients and healthy controls. GG and GA genotype frequencies were 78% and 22%, respectively, in periodontitis patients, while A and G allele frequencies were 11% and 89%. In healthy people, genotype frequencies of GG and GA were 92% and 8% and the frequencies of alleles A and G were 4% and 96%. No significant differences in genotype or allele distribution were revealed between the groups. There was no association between TNF-α G-308A SNP and periodontitis susceptibility in Indonesian males.
|Number of pages
|Journal of International Dental and Medical Research
|Published - 2020
- gene polymorphism
- Tumor necrosis factor alpha