TY - JOUR
T1 - RELA mRNA Expression in Epithelial Ovarian Cancer
T2 - Correlation with rs11820062 Gene Variant
AU - Saraswati, Benedikta D.
AU - Suryandari, Dwi A.
AU - Kodariah, Ria
AU - Sukmawati, Dewi
AU - Yunaini, Luluk
AU - Rustamadji, Primariadewi
AU - Sari, Puji
N1 - Funding Information:
We would especially like to thank the administrative and laboratory staff of the Biobank Research-IMERI FMUI and Anatomical Pathology FMUI for their assistance in obtaining the samples used in this study. We also would like to thank PUTI Q2 Grant year 2020 from the University of Indonesia who funded this study.
Publisher Copyright:
© 2023 Indonesian Society of Obstetrics and Gynecology. All rights reserved.
PY - 2023/4
Y1 - 2023/4
N2 - Objective: To examine the distribution of RELA rs11820062 and its correlation to i mRNA expression in low-grade and high-grade EOC’s patients from Dr. Cipto Mangunkusumo General hospital, Indonesia. Methods: This study is cross-sectional with a total of 65 healthy subjects and 80 ovarian biopsies (15 ovarian cysts as expression calibrators, 36 low-grade EOC, and 29 high-grade EOC) were used in this study. The distribution of genotypes and alleles was analyzed using ARMS PCR. The mRNA expressions of RELA were determined by real-time polymerase chain reaction (qPCR) analysis. Results: There was no significant difference between genotype and allele distributions for RELA rs11820062 in normal and case group. RELA relative mRNA expression was significantly higher in low-grade and high-grade EOC compared to in ovarian cysts (p<0.01). RELA rs11820062 CC genotype correlated to higher RELA mRNA relative expression and the TT genotype of RELA rs11820062 correlated with lower RELA mRNA relative expression in low-grade and high-grade EOC. Conclusion: C allele in rs11820062 caused an increased expression of RELA mRNA, which individuals with CC genotype correlated with higher RELA expression in low-grade and high-grade EOC. In contrast, individuals with the T allele of RELA rs11820062 had a protective effect against EOC risk because the RELA TT genotype tended to have a lower RELA mRNA expression in EOC.
AB - Objective: To examine the distribution of RELA rs11820062 and its correlation to i mRNA expression in low-grade and high-grade EOC’s patients from Dr. Cipto Mangunkusumo General hospital, Indonesia. Methods: This study is cross-sectional with a total of 65 healthy subjects and 80 ovarian biopsies (15 ovarian cysts as expression calibrators, 36 low-grade EOC, and 29 high-grade EOC) were used in this study. The distribution of genotypes and alleles was analyzed using ARMS PCR. The mRNA expressions of RELA were determined by real-time polymerase chain reaction (qPCR) analysis. Results: There was no significant difference between genotype and allele distributions for RELA rs11820062 in normal and case group. RELA relative mRNA expression was significantly higher in low-grade and high-grade EOC compared to in ovarian cysts (p<0.01). RELA rs11820062 CC genotype correlated to higher RELA mRNA relative expression and the TT genotype of RELA rs11820062 correlated with lower RELA mRNA relative expression in low-grade and high-grade EOC. Conclusion: C allele in rs11820062 caused an increased expression of RELA mRNA, which individuals with CC genotype correlated with higher RELA expression in low-grade and high-grade EOC. In contrast, individuals with the T allele of RELA rs11820062 had a protective effect against EOC risk because the RELA TT genotype tended to have a lower RELA mRNA expression in EOC.
KW - epithelial ovarian cancer
KW - NF-kB
KW - RELA
KW - rs11820062
UR - http://www.scopus.com/inward/record.url?scp=85162836239&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85162836239
SN - 2338-6401
VL - 11
SP - 112
EP - 118
JO - Indonesian Journal of Obstetrics and Gynecology
JF - Indonesian Journal of Obstetrics and Gynecology
IS - 2
ER -