Reduced endothelial cell migratory signal production byendometrial explants from women using norplantcontraception

Bekti Subakir, Wachyu Hadisaputra, Siregar Budiningsih, Dewi Irawati S. Santoso, Cornain Santoso, Affandi Biran

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19 Citations (Scopus)


Bleeding problems can be one of the major reasons for women to discontinue the use of hormonal contraceptives.Causes of endometrial bleeding can include disturbances in endometrial regeneration and angiogenesis. Endothelial cells migrate and proliferate rapidly as part of the angiogenic process under the influence of appropriate stimuli.The aim of this study is to investigate the production of endothelial cell migratory signals by endometrial explants from women receiving Norplant and to compare it to that of those with a normal menstrual cycle. The subjects were selected from Norplant users with an exposure of 3-9 months. The endothelial cell migratory signal production was assayed using the Folkman method (1989), modified by Rogers (1992). Blood serum concentrations of oestradiol,progesterone and sex hormone binding globulin were monitored for 2 weeks prior to endometrial biopsy.Endothelial cell migration toward endometrial explants of 30 women as control and 46 Norplant acceptors was assayed. The results showed that endothelial cell migratory activity toward endometrial explants from the control group was significantly higher than toward those from Norplant acceptors (z = -3.89, P < 0.001). There were no differences between endometrial endothelial cell migratory activities in Norplant acceptors with bleeding or withoutbleeding problems.

Original languageEnglish
Pages (from-to)2579-2583
Number of pages5
JournalHuman Reproduction
Issue number10
Publication statusPublished - Oct 1995


  • Angiogenesis
  • Endometrial bleeding
  • Endothelial cell
  • Levonorgestrel implant


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