Realization and testing of lab-on-chip for human lung replication

This research realised 3D constructs so called lab-on-chip as pathways to study human cell under a controlled environment. The chip is a sandwich of two microchannels with microporous membrane in between to mimic human lung system. The challenge is the fabrication of each micro part and assembles them becoming a functional micro system. Firstly, the session was preceded by realization of 20mm x 0.5mm x 0.3mm (length x width x thickness) channels. Later on, porous membrane with size of 100μm x 100μm arranged as arrays were also realised using micro milling apparatus. Eventually, the system was tested by employing 300ml/h propanol and water in each channel. The diffusion of propanol into water channel was validated by gas chromatography (GC) apparatus to validate the functionality of the system.