Real-time PCR for dihydrofolate reductase gene single-nucleotide polymorphisms in Plasmodium vivax isolates

Sara Brega, Frédérique De Monbrison, Carlo Severini, Rachanee Udomsangpetch, Inge Sutanto, Paul Ruckert, François Peyron, Stéphane Picot

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Mutations in the dhfr gene of Plasmodium vivax (pvdhfr) are associated with resistance to the antifolate antimalarial drugs. Polymorphisms in the pvdhfr gene were assessed by hybridization probe technology on the LightCycler instrument with 134 P. vivax-infected blood samples from Turkey (n = 24), Azerbaijan (n = 39), Thailand (n = 16), Indonesia (n = 53), and travelers (n = 19). Double mutations (S58R and S117N) or quadruple mutations (F57L/I, S58R, T61M, and S117N) in the pvdhfr genes were found in all Thai samples (100%). pvdhfr mutant-type alleles were significantly more common in samples from travelers (42%) than in those from patients from Indonesia (5%). Surprisingly, the pvdhfr single-mutation allele (S117N) was identified at a high frequency in parasites from Turkey and Azerbaijan (71 and 36%, respectively), where sulfadoxine-pyrimethamine is not recommended for the treatment of P. vivax malaria by the World Health Organization and the Malaria National Programs.

Original languageEnglish
Pages (from-to)2581-2587
Number of pages7
JournalAntimicrobial Agents and Chemotherapy
Volume48
Issue number7
DOIs
Publication statusPublished - 1 Jul 2004

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