Radiolabeling technique of silver nanoparticles (AgNPs) with iodine-131 radionuclide

U. N. Sholikhah, R. R. Syahdi, S. E. Permatasari, E. Sarmini, Sriyono, T. Widyaningrum

Research output: Contribution to journalArticlepeer-review


Radiotherapy is an effective cancer therapy, where a certain dose of radiation is aimed specifically at target and is unaffecting to normal tissue. A selective radionuclide must be attached to the specific targeted organ. In this research, silver nanoparticles (AgNPs) were labeled with radionuclide of iodine-131 (131I) to be used in radiotherapy. Silver nanoparticles were synthesized using silver nitrate 0.0005 M, sodium borohydride 0.002 M, polyvinylpyrrolidone 0.3 % (w/v) and natrium chloride 1.5 M, and then followed by purification by centrifugation. Characterization was carried out with UV-Vis spectrophotometer, transmission electron microscope, particle size analyzer, and zeta-sizer. The results show that the maximum absorbance is on a wavelength of 398 nm, spherical shape with a diameter of 10 nm, polydispersity index of 0.455, and zeta potential value of-8 mV. The radiolabeling was done by adding sodium iodide-131 which had been oxidized by chloramine-T and immobilized in AgNPs colloidal solution, and the activity was then measured using a dose calibrator. The identification of radionuclide showed that the sample was free of impurities. The optimum system of radiochemical purity was obtained using Whatman 1 paper strip as the stationary phase and a mixture of methanol: water: ammonium acetate (1:1:1) as the mobile phase, which gave 96 % purity. This method is suitable for radiolabeling AgNPs with 131I to be used for radiotherapy.

Original languageEnglish
Pages (from-to)63-68
Number of pages6
JournalAtom Indonesia
Issue number2
Publication statusPublished - 1 Aug 2020


  • Gamma counter
  • Radiochemical purity
  • Radionuclide purity
  • Radiotheraphy


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