Radical scavenging activity of extract, fraction and chemical compound from Calophyllum sclerophyllum vesq. stembark by using 1,1-diphenyl-2-picryl Hydrazil (DPPH)

Antioxidant activity by DPPH methods of ethanolic extract, fractions from Calophyllum sclerophyllum Vesq stembark, and the chemical compound from the active fraction as antioxidant has been measured in this research. Extraction was done by maceration methods with ethanol 70% and then partition by n-hexane, ethyl acetate and n-buthanol. Then antioxidant activity assays of ethanolic extract and fractions by DPPH methods and using quercetin as positive control. The fraction that have antioxidant activity more over purified by colomn chromatography and recrystalization, and the pure compounds were elucidated by spectroscopic analysis and measured that antioxidant activity. The ethanolic extract have high antioxidant activity with IC₅₀ 5,96 ppm, and also ethyl acetate fraction and n-buthanol extract with IC₅₀ 3,03 and 3,89 ppm. The IC₅₀ of quercetin value at 1,73 ppm. Two pure compounds have been isolated from ethyl acetate fraction, CSL 1 was chromanon acid (Isoapetalic acid) and CSL 2 was flavonoid (Astilbin), but only CSL 2 that showed activity as antioxidant, with IC₅₀ 7, 24 ppm. Those compounds not yet reported before from this species.