Radical scavenging activity assay and red fluorescence microscopy studies: Antioxidant properties of selected young and mature leaves for application in pharmaceutical industry

Dalina Daud; Syee Chee Yang; Cynthia C. Balaja; Fairuzeta Ja’afar; Hartini M. Yasin; Eny Kusrini; Wuwuh W. Prihandini; Anwar Usman

doi:10.5109/4055222

Radical scavenging activity assay and red fluorescence microscopy studies: Antioxidant properties of selected young and mature leaves for application in pharmaceutical industry

Dalina Daud, Syee Chee Yang, Cynthia C. Balaja, Fairuzeta Ja’afar, Hartini M. Yasin, Eny Kusrini, Wuwuh W. Prihandini, Anwar Usman

Department of Chemical Engineering

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

A fast screening method for antioxidant capacity of plant leaves based on red fluorescence was studied. The red fluorescence of young and matured leaves of A. occidentale, M. indica, S. dulcis, belonging to Anacardiaceae, and P. betle Linn., P. sarmentosum, and P. crocatum of Piperaceae family using fluorescence imaging microscopy was investigated. The total antioxidant capacity of methanol extracts of plant leaves was determined using the 2,2-diphenyl-1-picrylhydrazine (DPPH) free radical scavenging assay. From young to matured leaves of the plants belonging to the Anacardiaceae and Piperaceae, the red fluorescence and the total antioxidant capacity show the same trend.

Original language	English
Pages (from-to)	216-220
Number of pages	5
Journal	Evergreen
Volume	7
Issue number	2
DOIs	https://doi.org/10.5109/4055222
Publication status	Published - Jun 2020

Keywords

Anacardiaceae
Antioxidant
DPPH assay
Piperaceae
Red fluorescence

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10.5109/4055222

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title = "Radical scavenging activity assay and red fluorescence microscopy studies: Antioxidant properties of selected young and mature leaves for application in pharmaceutical industry",

abstract = "A fast screening method for antioxidant capacity of plant leaves based on red fluorescence was studied. The red fluorescence of young and matured leaves of A. occidentale, M. indica, S. dulcis, belonging to Anacardiaceae, and P. betle Linn., P. sarmentosum, and P. crocatum of Piperaceae family using fluorescence imaging microscopy was investigated. The total antioxidant capacity of methanol extracts of plant leaves was determined using the 2,2-diphenyl-1-picrylhydrazine (DPPH) free radical scavenging assay. From young to matured leaves of the plants belonging to the Anacardiaceae and Piperaceae, the red fluorescence and the total antioxidant capacity show the same trend.",

keywords = "Anacardiaceae, Antioxidant, DPPH assay, Piperaceae, Red fluorescence",

author = "Dalina Daud and Yang, {Syee Chee} and Balaja, {Cynthia C.} and Fairuzeta Ja{\textquoteright}afar and Yasin, {Hartini M.} and Eny Kusrini and Prihandini, {Wuwuh W.} and Anwar Usman",

year = "2020",

month = jun,

doi = "10.5109/4055222",

language = "English",

volume = "7",

pages = "216--220",

journal = "Evergreen",

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T1 - Radical scavenging activity assay and red fluorescence microscopy studies

T2 - Antioxidant properties of selected young and mature leaves for application in pharmaceutical industry

AU - Daud, Dalina

AU - Yang, Syee Chee

AU - Balaja, Cynthia C.

AU - Ja’afar, Fairuzeta

AU - Yasin, Hartini M.

AU - Kusrini, Eny

AU - Prihandini, Wuwuh W.

AU - Usman, Anwar

PY - 2020/6

Y1 - 2020/6

N2 - A fast screening method for antioxidant capacity of plant leaves based on red fluorescence was studied. The red fluorescence of young and matured leaves of A. occidentale, M. indica, S. dulcis, belonging to Anacardiaceae, and P. betle Linn., P. sarmentosum, and P. crocatum of Piperaceae family using fluorescence imaging microscopy was investigated. The total antioxidant capacity of methanol extracts of plant leaves was determined using the 2,2-diphenyl-1-picrylhydrazine (DPPH) free radical scavenging assay. From young to matured leaves of the plants belonging to the Anacardiaceae and Piperaceae, the red fluorescence and the total antioxidant capacity show the same trend.

AB - A fast screening method for antioxidant capacity of plant leaves based on red fluorescence was studied. The red fluorescence of young and matured leaves of A. occidentale, M. indica, S. dulcis, belonging to Anacardiaceae, and P. betle Linn., P. sarmentosum, and P. crocatum of Piperaceae family using fluorescence imaging microscopy was investigated. The total antioxidant capacity of methanol extracts of plant leaves was determined using the 2,2-diphenyl-1-picrylhydrazine (DPPH) free radical scavenging assay. From young to matured leaves of the plants belonging to the Anacardiaceae and Piperaceae, the red fluorescence and the total antioxidant capacity show the same trend.

KW - Anacardiaceae

KW - Antioxidant

KW - DPPH assay

KW - Piperaceae

KW - Red fluorescence

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DO - 10.5109/4055222

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EP - 220

JO - Evergreen

JF - Evergreen

IS - 2

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Radical scavenging activity assay and red fluorescence microscopy studies: Antioxidant properties of selected young and mature leaves for application in pharmaceutical industry

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Keywords

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