Quercetin concentration and total flavonoid content of anti-atherosclerotic herbs using aluminum chloride colorimetric assay

Dewi Tristantini, Riska Amalia

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Atherosclerosis is plaque formation in the arteries which will further develop into plaque, narrowing and shocking the artery. Continuous plaque formation leads to acute and chronic inflammation and soon develop into atherosclerosis. A combination of herbs in form of drink, consist of tanjung leaves (Mimusops elengi L.), starfruit leaves (Averrhoa carambola L.), and temulawak (Curcuma xanthorrhiza R.) is empirically believed to be anti-atherosclerotic; because it contains quercetin that initiate anti-inflammatory activity. Quercetin concentration test was conducted using an aluminum chloride colorimetric assay with variations in particle size (simplicia, 60<D<16 mesh powder, and D≤60 mesh powder) and herb mass, which further extracted in reflux and infusion method. Herbs with 60<D<16 mesh size and D≤60 mesh powder were extracted by infusion in 200 mL water, and leaf herbs were extracted by reflux in 600 mL water. The results show that increases in extracted herb mass related to the increasing in quercetin concentration. For future product commercialization purpose, quercetin concentration in leaves needs to be equal to its concentration within the range of particle size variation. Quercetin concentration in the leaves (459.91 ppm) was almost equal to that for 2.4 gram herbs in 60<D<16 mesh powder (489.09 ppm). The highest total flavonoid content was achieved in 2.4 gram 60<D<16 mesh herb powder with 41.508 mg QE (Quercetin Equivalent)/g. Therefore, it can be concluded that the anti-atherosclerotic herbs contain quercetin.

Original languageEnglish
Title of host publication4th Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2019
EditorsKenny Lischer, Tomy Abuzairi, Siti Fauziyah Rahman, Misri Gozan
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735419445
DOIs
Publication statusPublished - 10 Dec 2019
Event4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019 - Padang, West Sumatera, Indonesia
Duration: 22 Jul 201924 Jul 2019

Publication series

NameAIP Conference Proceedings
Volume2193
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference4th International Symposium of Biomedical Engineering�s Recent Progress in Biomaterials, Drugs Development, Health, and Medical Devices, ISBE 2019
CountryIndonesia
CityPadang, West Sumatera
Period22/07/1924/07/19

Keywords

  • Atherosclerosis
  • Averrhoa carambola L.
  • Curcuma xanthorrhiza R
  • Flavonoid
  • Mimusops elengi L.
  • Quercetin

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