Quantitative real-time pcr of cps type 1, 2 and 5 of enterococcus faecalis and candida albican isolated from infected root canal of subject requiring endodontic treatment

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Abstract

Enterococcus faecalis and Candida albicans is a normal microflora in the oral cavity but can also be isolated from the root canal infection of primary and persistence cases. Aim: The purpose of this research to investigate the amount of both E. faecalis and C. albicans and the capsular polysaccharide (cps) type isolated from root canal samples of Indonesian requiring endodontic treatment. One hundred and fourteen outpatients attended three private hospitals in Jakarta Indonesia for endodontic treatment provided root canal samples. The clinical samples were collected from infected root canal teeth and grown on Chrom agar plate, while PCR was used to confirmed the species as well as their cps types. Additionally, the proportion of both E. faecalis and C. albicans in infected root canal was analyzed by using qPCR The number of E. faecalis and C. albicans in endodontic retreatment group almost doubled compared to the group of primary endodontic treatment. Statistical test results showed a non-significant, P> 0.05. The correlation between the relative amounts of C. albicans and E. faecalis showed a strong linear relationship for groups of primary endodontic treatment with the value of 0.624 (p <0.001) as well as overall relations with the value of 0.514 (p <0.001). Endodontic retreatment group also has a strong enough relationship with a value of 0.399, p = 0.003. Overall cps 1 is more dominant (76,3,0%) compared to the combination of cps 1-2 (9,7%) and cps 1-5 (14%). C. albicans and E. faecalis can be found as much as persistent microorganisms in the root canal endodontic retreatment cases. The average relative amount of E. faecalis in endodontic retreatment group was much higher while C. albicans is higher in primary groups. While the expression of capsular polysaccharide (cps) type 1, not encapsulated, dominates the encapsulated type cps 2 and 5.

Original languageEnglish
Pages (from-to)157-163
Number of pages7
JournalJournal of International Dental and Medical Research
Volume9
Issue number3
Publication statusPublished - 1 Jan 2016

Keywords

  • C. albican
  • Cps 1, 2, 5, primary endodontic treatment and endodontic retreatment
  • E.faecalis

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