Quantitative measurement of porphyromonas gingivalis and treponema denticola levels on dental plaque and its relationship with periodontal status and coronary heart disease

Coronary heart disease (CHD) is a narrowing of coronary artery due to plaque build-up, a process called atherosclerosis. Chronic infection such as chronic periodontitis is a risk factor for atherosclerosis. Porphyromonas gingivalis and Treponema denticola are linked to chronic periodontitis. To analyse quantitative difference of P.gingivalis and T.denticola on dental plaque and its relationship with periodontal status of CHD patients and control. Methods: Periodontal status of 66 CHD patients and 40 controls was obtained. Subgingival plaque was isolated. P.gingivalis and T.denticola level were measured using real-time PCR. P.gingivalis level of CHD patients (6.95(1.77) log10 CFU/ml) was significantly different from control (6.12(1.32) log10 CFU/ml). T.denticola level of CHD patients (5.26(2.27) log10 CFU/ml) was significantly different from control (3.59(1.93) log10 CFU/ml). P.gingivalis was linked to the pocket depth (PD) of CHD patient (p=0.03). T.denticola was not significntly associated with any periodontal status (p < 0.05). P.gingivalis and T.denticola levels of CHD patients were higher than control. P.gingivalis level is not linked to any periodontal status, except for pocket depth of CHD patient. T.denticola was not associated with any periodontal status.