Quantitative Analysis of Selenomonas noxia in Periodontitis Patients via Two Different Sample Collection Methods

Nadia Regina S. Kodrat, Hari Sunarto, Y. Soeroso, Boy M. Bachtiar

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Selenomonas noxia is a bacterium that is a putative periodontal pathogen found in the gingival crevice and periodontal pocket and is suspected to have a significant role in the initiation and progression of periodontitis. This study aimed to analyse the relationship between S. noxia bacterium quantity in healthy and periodontitis patients, based on clinical findings marked by periodontal pocket depth (PD), periodontal bleeding index (PBI), and clinical attachment loss (CAL) via two sample collection methods using paper points (PP) and Gracey curettes (GC). The samples with PD, PBI, and CAL measured, were collected using PP and GC in each site, resulting in 35 samples in total for each collection method. The S. noxia strain was obtained from the American Type Culture Collection (ATCC 51893) and used to create a standard curve in real-time polymerase chain reaction (qPCR). This study shows that the quantity of S. noxia numbers were higher (PP, P=0,01; GC, P=0,03) in periodontitis subjects than in healthy subjects. The difference in S. noxia numbers also observed in CAL 0 mm and <5 mm, and 0 and CAL ≥5 mm, also in sulcus (1–3 mm) and PD <6 mm groups. These results show that patients with periodontitis had higher S. noxia levels than healthy subjects did. However, there was no positive correlation in the PBI category and S. noxia amount, and in bacterium load in PP and GC samples. Thus, S. noxia can be detected with either PP or GC samples.

Original languageEnglish
Pages (from-to)1320-1325
Number of pages6
JournalJournal of International Dental and Medical Research
Issue number4
Publication statusPublished - 2020


  • dental plaque
  • Periodontitis
  • Selenomonas noxia


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