TY - JOUR
T1 - Quantitative Analysis of Selenomonas noxia in Periodontitis Patients via Two Different Sample Collection Methods
AU - Kodrat, Nadia Regina S.
AU - Sunarto, Hari
AU - Soeroso, Y.
AU - Bachtiar, Boy M.
N1 - Funding Information:
All authors have made substantive contribution to this study and/or manuscript, and all have reviewed the final paper prior to its submission. We would like to acknowledge the support of Dental Teaching Hospital and Oral Biology Laboratory, Faculty of Dentistry, Universitas Indonesia staffs, also PITTA grant for funding this study (grant number: 2178/UN2.R3.1/HKP.05.00/2018)
Publisher Copyright:
© 2020. All Rights Reserved.
PY - 2020
Y1 - 2020
N2 - Selenomonas noxia is a bacterium that is a putative periodontal pathogen found in the gingival crevice and periodontal pocket and is suspected to have a significant role in the initiation and progression of periodontitis. This study aimed to analyse the relationship between S. noxia bacterium quantity in healthy and periodontitis patients, based on clinical findings marked by periodontal pocket depth (PD), periodontal bleeding index (PBI), and clinical attachment loss (CAL) via two sample collection methods using paper points (PP) and Gracey curettes (GC). The samples with PD, PBI, and CAL measured, were collected using PP and GC in each site, resulting in 35 samples in total for each collection method. The S. noxia strain was obtained from the American Type Culture Collection (ATCC 51893) and used to create a standard curve in real-time polymerase chain reaction (qPCR). This study shows that the quantity of S. noxia numbers were higher (PP, P=0,01; GC, P=0,03) in periodontitis subjects than in healthy subjects. The difference in S. noxia numbers also observed in CAL 0 mm and <5 mm, and 0 and CAL ≥5 mm, also in sulcus (1–3 mm) and PD <6 mm groups. These results show that patients with periodontitis had higher S. noxia levels than healthy subjects did. However, there was no positive correlation in the PBI category and S. noxia amount, and in bacterium load in PP and GC samples. Thus, S. noxia can be detected with either PP or GC samples.
AB - Selenomonas noxia is a bacterium that is a putative periodontal pathogen found in the gingival crevice and periodontal pocket and is suspected to have a significant role in the initiation and progression of periodontitis. This study aimed to analyse the relationship between S. noxia bacterium quantity in healthy and periodontitis patients, based on clinical findings marked by periodontal pocket depth (PD), periodontal bleeding index (PBI), and clinical attachment loss (CAL) via two sample collection methods using paper points (PP) and Gracey curettes (GC). The samples with PD, PBI, and CAL measured, were collected using PP and GC in each site, resulting in 35 samples in total for each collection method. The S. noxia strain was obtained from the American Type Culture Collection (ATCC 51893) and used to create a standard curve in real-time polymerase chain reaction (qPCR). This study shows that the quantity of S. noxia numbers were higher (PP, P=0,01; GC, P=0,03) in periodontitis subjects than in healthy subjects. The difference in S. noxia numbers also observed in CAL 0 mm and <5 mm, and 0 and CAL ≥5 mm, also in sulcus (1–3 mm) and PD <6 mm groups. These results show that patients with periodontitis had higher S. noxia levels than healthy subjects did. However, there was no positive correlation in the PBI category and S. noxia amount, and in bacterium load in PP and GC samples. Thus, S. noxia can be detected with either PP or GC samples.
KW - dental plaque
KW - Periodontitis
KW - Selenomonas noxia
UR - http://www.scopus.com/inward/record.url?scp=85103515978&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85103515978
SN - 1309-100X
VL - 13
SP - 1320
EP - 1325
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 4
ER -