Purification of recombinant protein apoptin from two cell host Bacillus subtilis 168 and Escherichia coli Bl21 Star

Ihsan Wiratama, Heri Hermansyah, Anondho Wijarnako, Amarila Malik, Raditya Imamul Khalid, Muhamad Sahlan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Cancer is a deadly disease so that the medicinal treatment constantly developed. Apoptin is a protein molecule that has potential to be used as a cancer drug because of its activity to induce cell death selectively to the cancer cells only. Cloning apoptin has been successfully performed by amplify gene using PCR with 12-histidine and 8-arginine to be added at C-terminal then ligated into plasmid pOXGW with Gateway system, and then expressed in Bacillus subtilis 168. Plasmids with pOXGW - apop - 12His8Arg can be expressed in B. subtilis. In this study, Bacillus subtilis carrying plasmid was produced with variations of xylose as substrate trigger on liquid medium and as a comparison, Escherichia coli Bl21 Star transformed with a plasmid pOGW - apop - 12His and then performed for purification of apoptin. The results showed that the recombinant apoptin obtain from B. subtilis 168 compared to Escherichia coli Bl21 Star is slightly higher, i.e. 568μg/ml and 421μg/ml, respectively.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
CountryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • Apoptin
  • Bacillus subtilis 168
  • Escherichia coli Bl21 Star
  • plasmid pOXGW - apop - 12His8Arg
  • POGW apop 12His
  • xylose

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