TY - JOUR
T1 - Protein profiling of Streptococcus mutans isolated from the surface of the tongue in children with early childhood caries
AU - Aldilavita, H.
AU - Gultom, F. P.
AU - Bachtiar, E. W.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - The pathogenesis of early childhood caries (ECC) is influenced by the virulence factors of Streptococcus mutans, a major etiological factor of dental caries protein profile. To analyze the differences in the protein profiles of S. mutans isolated from the surface of the tongue in ECC and caries-free children. Saliva samples were collected from 32 children (16 with ECC and 16 caries-free). Protein profiling was performed using the SDS-PAGE method. Examination of protein bands on the polyacrylamide gel revealed differences in the frequencies of several S. mutans proteins. The molecular weights of the proteins observed in the ECC and free caries children were as follows: 13, 29, 39, 41.3, 74, and 94.5 kDa. The proteins virulent to ECC (41.3 kDa and 74 kDa) were more frequently expressed in children with ECC when compared to the caries-free children. Differences in the protein profiles of S. mutans isolated from the tongue were observed between the ECC and caries-free children in this study.
AB - The pathogenesis of early childhood caries (ECC) is influenced by the virulence factors of Streptococcus mutans, a major etiological factor of dental caries protein profile. To analyze the differences in the protein profiles of S. mutans isolated from the surface of the tongue in ECC and caries-free children. Saliva samples were collected from 32 children (16 with ECC and 16 caries-free). Protein profiling was performed using the SDS-PAGE method. Examination of protein bands on the polyacrylamide gel revealed differences in the frequencies of several S. mutans proteins. The molecular weights of the proteins observed in the ECC and free caries children were as follows: 13, 29, 39, 41.3, 74, and 94.5 kDa. The proteins virulent to ECC (41.3 kDa and 74 kDa) were more frequently expressed in children with ECC when compared to the caries-free children. Differences in the protein profiles of S. mutans isolated from the tongue were observed between the ECC and caries-free children in this study.
UR - http://www.scopus.com/inward/record.url?scp=85054488308&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032032
DO - 10.1088/1742-6596/1073/3/032032
M3 - Conference article
AN - SCOPUS:85054488308
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032032
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -