TY - JOUR
T1 - Protective effect of yacon leaves extract (Smallanthus sonchifolius(Poepp.) H. Rob) through antifibrosis, anti-inflammatory, and antioxidant mechanisms toward diabetic nephropathy
AU - Widowati, Wahyu
AU - Tjokropranoto, Rita
AU - Onggowidjaja, Philips
AU - Widya Kusuma, Hanna
AU - Wijayanti, Cahyaning
AU - Marthania, Meganita
AU - Yati, Afif
AU - Rizal, Rizal
N1 - Publisher Copyright:
© 2023 Wolters Kluwer Medknow Publications. All rights reserved.
PY - 2023/5/1
Y1 - 2023/5/1
N2 - Background and purpose: Diabetic nephropathy (DN) is a chronic kidney failure, which may lead to fatality. Mesangial cell proliferation, renal inflammation, stress oxidative, and fibrosis are involved in DN progression. Yacon leaves (Smallanthus sonchifolius (Poepp.) H. Rob.) contains large amounts of phenolic compounds and it has the ability to inhibit oxidative stress, inflammation, and fibrosis. Considering the potential of yacon leaves extract (YLE), it may be used for DN treatment. This research aimed to elucidate YLE's potential as anti-DN through anti-inflammatory, antioxidant, and antifibrosis mechanisms. Experimental approach: Mesangial cells were induced by glucose 20 mM for 5 days and treated with YLE concentrations as much as 5, 10, and 50 μg/mL. TGF-β1, TNF-α, and MDA levels were measured using the ELISA method. SMAD2, SMAD3, SMAD4, and SMAD7 gene expressions were analyzed using the qRT-PCR method. Findings/Results: YLE at 5, 10, and 50 μg/mL could reduce the levels of TGF-β1, TNF-α, and MDA compared with the DN cells model. YLE could reduce gene expressions of SMAD2, SMAD3, and SMAD4 and increase SMAD7 expression. Conclusion and implications: YLE potentially mitigated diabetic nephropathy through antifibrosis, anti-inflammatory, and antioxidant capacities.
AB - Background and purpose: Diabetic nephropathy (DN) is a chronic kidney failure, which may lead to fatality. Mesangial cell proliferation, renal inflammation, stress oxidative, and fibrosis are involved in DN progression. Yacon leaves (Smallanthus sonchifolius (Poepp.) H. Rob.) contains large amounts of phenolic compounds and it has the ability to inhibit oxidative stress, inflammation, and fibrosis. Considering the potential of yacon leaves extract (YLE), it may be used for DN treatment. This research aimed to elucidate YLE's potential as anti-DN through anti-inflammatory, antioxidant, and antifibrosis mechanisms. Experimental approach: Mesangial cells were induced by glucose 20 mM for 5 days and treated with YLE concentrations as much as 5, 10, and 50 μg/mL. TGF-β1, TNF-α, and MDA levels were measured using the ELISA method. SMAD2, SMAD3, SMAD4, and SMAD7 gene expressions were analyzed using the qRT-PCR method. Findings/Results: YLE at 5, 10, and 50 μg/mL could reduce the levels of TGF-β1, TNF-α, and MDA compared with the DN cells model. YLE could reduce gene expressions of SMAD2, SMAD3, and SMAD4 and increase SMAD7 expression. Conclusion and implications: YLE potentially mitigated diabetic nephropathy through antifibrosis, anti-inflammatory, and antioxidant capacities.
KW - Diabetic nephropathy
KW - MDA
KW - SMADs
KW - TGF-β1
KW - TNF-α
KW - Yacon leaves extract.
UR - http://www.scopus.com/inward/record.url?scp=85151613678&partnerID=8YFLogxK
U2 - 10.4103/1735-5362.371589
DO - 10.4103/1735-5362.371589
M3 - Article
AN - SCOPUS:85151613678
SN - 1735-5362
VL - 18
SP - 336
EP - 345
JO - Research in Pharmaceutical Sciences
JF - Research in Pharmaceutical Sciences
IS - 3
ER -