TY - JOUR
T1 - Protective Effect of Phaleria macrocarpa Water Extract (Proliverenol) against Carbon Tetrachloride-Induced Liver Fibrosis in Rats
T2 - Role of TNF-α and TGF-β1
AU - Sundari, Nanik
AU - Vivian, null
AU - Louisa, Melva
AU - Wardhani, Bantari W.
AU - Tjandrawinata, Raymond R.
PY - 2018/1/1
Y1 - 2018/1/1
N2 -
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to elucidate its mechanism of action. Male Sprague-Dawley rats were treated with carbon tetrachloride (CCl
4
) for 8 weeks to induce liver fibrosis. Rats were randomly divided into 6 groups (n=5), i.e., control group, CCl4 group, CCl4 + NAC group, CCl4 + various doses of water extract of Phaleria macrocarpa (50, 100, and 150 mg/kg body weight). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), liver histopathology, malondialdehyde (MDA), ratio GSH/GSSG, Tumor Necrosis Factor- (TNF-) α, and Transforming Growth Factor- (TGF-) β
1
were analyzed. This study demonstrated that water extract of Phaleria macrocarpa and NAC significantly protected CCl
4
-induced liver injury as demonstrated by reduced AST, ALT, ALP, and fibrosis percentage compared with the CCl
4
-only group. In addition, water extract of Phaleria macrocarpa and NAC significantly reduced the levels of MDA, TNF-α, and TGF-β
1
as well as increasing the ratio of GSH/GSSG. Water extract of Phaleria macrocarpa prevents CCl
4
-induced fibrosis in rats. The prevention of liver fibrosis was at least in part through its antioxidant and anti-inflammatory activities and through its capacity to inhibit hepatic stellate cells (HSC) activation by reducing fibrogenic cytokine TGF-β
1
AB -
Phaleria macrocarpa is one of the Indonesian herbal plants which has been shown to have a hepatoprotective effect. This study was conducted to evaluate the protective effect of water extract of mahkota dewa (Phaleria macrocarpa) in liver fibrosis and to elucidate its mechanism of action. Male Sprague-Dawley rats were treated with carbon tetrachloride (CCl
4
) for 8 weeks to induce liver fibrosis. Rats were randomly divided into 6 groups (n=5), i.e., control group, CCl4 group, CCl4 + NAC group, CCl4 + various doses of water extract of Phaleria macrocarpa (50, 100, and 150 mg/kg body weight). Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), liver histopathology, malondialdehyde (MDA), ratio GSH/GSSG, Tumor Necrosis Factor- (TNF-) α, and Transforming Growth Factor- (TGF-) β
1
were analyzed. This study demonstrated that water extract of Phaleria macrocarpa and NAC significantly protected CCl
4
-induced liver injury as demonstrated by reduced AST, ALT, ALP, and fibrosis percentage compared with the CCl
4
-only group. In addition, water extract of Phaleria macrocarpa and NAC significantly reduced the levels of MDA, TNF-α, and TGF-β
1
as well as increasing the ratio of GSH/GSSG. Water extract of Phaleria macrocarpa prevents CCl
4
-induced fibrosis in rats. The prevention of liver fibrosis was at least in part through its antioxidant and anti-inflammatory activities and through its capacity to inhibit hepatic stellate cells (HSC) activation by reducing fibrogenic cytokine TGF-β
1
UR - http://www.scopus.com/inward/record.url?scp=85058888325&partnerID=8YFLogxK
U2 - 10.1155/2018/2642714
DO - 10.1155/2018/2642714
M3 - Article
AN - SCOPUS:85058888325
VL - 2018
JO - Journal of Toxicology
JF - Journal of Toxicology
SN - 1687-8191
M1 - 2642714
ER -