Protective Effect of Ethanolic Extract of Jati Belanda (Guazuma ulmifolia L.) by Inhibiting Oxidative Stress and Inflammatory Processes in Cisplatin-induced Nephrotoxicity in Rats

The accumulation of high concentrations of cisplatin in the kidneys may cause nephrotoxicity. Cisplatin-induced nephrotoxicity happens through the inflammation of the tubules, and apoptosis, necrosis, and vascular defects. Jati Belanda (Guazuma ulmifolia L.) leave contain various natural compounds and the ethanolic extract of Jati Belanda decreased Reactive Oxygen Species (ROS) level in diabetic glomerulosclerosis by in vitro study. According to the author’s knowledge, no comprehensive work was dedicated to nephroprotective effect of Jati Belanda ethanolic extract (EEJB) in cisplatin-induced nephrotoxic rat. Two doses of EEJB 300 mg/kg bw, EEJB 600 mg/kg bw were administered in nephrotoxicity rats by inducing cisplatin 8 mg/kg bw for three days before EEJB treatment. The measurement of MDA and CRE were done using colorimetric methods while IFN-γ. IL-1β, and TNF-α expression was done by using immunochemistry (IHC) assay. The results of this study showed that EEJB 300 and 600 mg/kg bw could decrease MDA and CRE level also IFN-γ. IL-1β, and TNF-α expression compared with positive control. Thus, ethanolic extract of Jati Belanda may give protective effect in patients being treated with chemotherapy using cisplatin but further toxicity effect and clinical trials studies are needed.