TY - JOUR
T1 - Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine
AU - Pawitan, Jeanne Adiwinata
AU - Leviana, Melisa
AU - Sukmawati, Dewi
AU - Liem, Isabella Kurnia
AU - Margiana, Ria
AU - Tarcisia, Twidy
N1 - Publisher Copyright:
© 2009-2017, JGPT. All Rights Reserved.
PY - 2017
Y1 - 2017
N2 - Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests. We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.
AB - Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests. We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.
KW - Culture waste
KW - Growth factor
KW - Mesenchymal stem cell
KW - Regenerative medicine
KW - Umbilical cord
UR - http://www.scopus.com/inward/record.url?scp=85056110777&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85056110777
SN - 0975-8542
VL - 9
JO - Journal of Global Pharma Technology
JF - Journal of Global Pharma Technology
IS - 7
ER -