Prospect of umbilical cord mesenchymal stem cell culture waste in regenerative medicine

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3 Citations (Scopus)

Abstract

Objective:to analyzevarious growth factor (GF) content in umbilical cord derived mesenchymal stem cell (UC-MSC) culture waste at harvest.Methods: We cultured UC- MSCs in complete medium, and collected the medium waste at harvests. We noted the time of harvests, and measured the concentrations of EGF, VEGF, NGF and Pl GF in the various medium wastes using ELISA method. We grouped GF concentrations according to various culture lengthsat harvest, and calculated themean values and standard deviations of the various GF. We compareddescriptively the GF concentrations at various culture lengths at harvests to conclude which condition yielded the overall highest GF concentration.Results: EGF, VEGF and PlGF concentrations were highest at harvest day-3, whileNGF concentration was the highest at harvest-day-5, respectively. Therefore, overall highest concentration of most GF was at harvest day 3.Conclusion:UC-MSC culture waste at harvest day-3 containedEGF, VEGF, NGF and PlGF, which might be used in regenerative medicine.

Original languageEnglish
JournalJournal of Global Pharma Technology
Volume9
Issue number7
Publication statusPublished - 1 Jan 2017

Keywords

  • Culture waste
  • Growth factor
  • Mesenchymal stem cell
  • Regenerative medicine
  • Umbilical cord

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