TY - JOUR
T1 - Proportion of Streptococcus mutans, Streptococcus sanguinis, and Candida albicans in Early Childhood Caries
T2 - Evaluation by qPCR
AU - Agustin, Tri Putriany
AU - Sutadi, Heriandi
AU - Bachtiar, Boy M.
AU - Rizal, Mochamad Fahlevi
N1 - Publisher Copyright:
© 2024 The Author(s).
PY - 2024
Y1 - 2024
N2 - Background: Early childhood caries (ECC) is one of the most common diseases in children. It is associated with oral microbiome dysbiosis, characterized by a reduced beneficial oral microbiome and an increased pathogenic oral microbiome. Objective: This study aims to detect and quantify S. mutans, S. sanguinis, and C. albicans in dental plaque samples from caries-free and ECC groups of children using the quantitative polymerase chain reaction (qPCR). Materials and Methods: Supragingival plaque samples were collected from 30 children aged 2 to 5 divided into three groups: 15 caries-free children, 7 ECC, and 8 S-ECC. The abundance of microorganisms was determined by qPCR. The obtained data were analyzed using one-way ANOVA (post hoc Bonferroni) and the Pearson and Spearman correlation tests. Results: The S-ECC group had a higher proportion of S. mutans and C. albicans. The proportion of S. sanguinis was higher in the ECC and had a positive correlation (P < 0.05) with S. mutans in this same group. Conclusion: The proportion of S. mutans, S. sanguinis, and C. albicans differed between the caries-free, ECC, and S-ECC groups, and they correlated with each other in the occurrence of ECC.
AB - Background: Early childhood caries (ECC) is one of the most common diseases in children. It is associated with oral microbiome dysbiosis, characterized by a reduced beneficial oral microbiome and an increased pathogenic oral microbiome. Objective: This study aims to detect and quantify S. mutans, S. sanguinis, and C. albicans in dental plaque samples from caries-free and ECC groups of children using the quantitative polymerase chain reaction (qPCR). Materials and Methods: Supragingival plaque samples were collected from 30 children aged 2 to 5 divided into three groups: 15 caries-free children, 7 ECC, and 8 S-ECC. The abundance of microorganisms was determined by qPCR. The obtained data were analyzed using one-way ANOVA (post hoc Bonferroni) and the Pearson and Spearman correlation tests. Results: The S-ECC group had a higher proportion of S. mutans and C. albicans. The proportion of S. sanguinis was higher in the ECC and had a positive correlation (P < 0.05) with S. mutans in this same group. Conclusion: The proportion of S. mutans, S. sanguinis, and C. albicans differed between the caries-free, ECC, and S-ECC groups, and they correlated with each other in the occurrence of ECC.
KW - Candida albicans
KW - Early childhood caries
KW - Pediatric dentistry
KW - qPCR
KW - Streptococcus mutans
KW - Streptococcus sanguinis
UR - http://www.scopus.com/inward/record.url?scp=85186554562&partnerID=8YFLogxK
U2 - 10.2174/0118742106290568240126040418
DO - 10.2174/0118742106290568240126040418
M3 - Article
AN - SCOPUS:85186554562
SN - 1874-2106
VL - 18
JO - Open Dentistry Journal
JF - Open Dentistry Journal
M1 - e18742106290568
ER -