The extraction process of propolis produces by-products named propolis wax tends unwanted by people. Antimicrobial and antioxidant properties of propolis wax can be utilized as an active substance in transparent soap. Propolis wax transparent soap produced in the quality of SNI 06-3532-1994, are not significantly different with propolis soap commercial that has been sold in the market. Then the antimicrobial activity of samples were tested using Total Plate Count method. The percentage inhibition of microbial origin hands of propolis wax transparent soap 1%, 1.5% and 2% respectively are 28.25%, 45.06% and 62.62%, while commercial Lifebuoy soap with the same test has microbial inhibition percentage of 53.54%. For organoleptic test results, in terms of appearance and scent soap panelists prefer the propolis wax soap 1%, while in terms of subtle impression and the resulting foam soap panelists preferred 2% propolis wax.