TY - JOUR
T1 - Promoter hypermethylation in progesterone receptor-A (PGR-A) and PGR-B gene decreased its mRNA expression in ovarian endometriosis
AU - Febri, Ririn Rahmala
AU - Muharam, R.
AU - Asmarinah, null
N1 - Funding Information:
We gratefully acknowledge Achmad Kemal Harzif and Catherine for their technical support. The publication of this manuscript is supported by Universitas Indonesia.
Publisher Copyright:
© 2019 Journal of International Dental and Medical Research.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The pathophysiology of endometriosis is not fully understood. It is believed that Progesterone Receptors (PGRs) play an important rule in the development of the disease. PGRs are expressed in human endometrial stromal. Reduction level of PGRs is related to the molecular basis of progesterone resistance in endometriosis. The aim of this study was to evaluate the association between methylation level of PGR-A and PGR-B gene and its mRNA expression in ovarian endometriosis. We used endometriotic tissues from 20 patients, aged 20-35 years, compared to endometrial tissues obtained from 20 normal women, age-matched to endometriosis group, who underwent micro-curettage to evaluate methylation status and mRNA expression of PGR-A and PGR-B gene. Methylation status of both PGRs was analyzed using MSP, and mRNA expression was investigated using qPCR. Methylation level of PGR-A (10,84%) and PGR-B (98,72%) in ovarian endometriosis were significantly different compared to control. The mRNA expression of PGR-A and PGR-B in ovarian endometriotic tissues significantly decreased by 2.35 and 6.37 folds compared to the normal endometrial tissues. Hypermethylation of PGR-B gene was correlated to mRNA expression, while methylation level of PGR-A had no correlation to mRNA expression. Promoter regions of PGR-A and -B genes in ovarian endometriosis are hypermethylated compared to controls. These hypermethylationmay reduce their expressions, hence might contribute to pathogenesis of endometriosis.
AB - The pathophysiology of endometriosis is not fully understood. It is believed that Progesterone Receptors (PGRs) play an important rule in the development of the disease. PGRs are expressed in human endometrial stromal. Reduction level of PGRs is related to the molecular basis of progesterone resistance in endometriosis. The aim of this study was to evaluate the association between methylation level of PGR-A and PGR-B gene and its mRNA expression in ovarian endometriosis. We used endometriotic tissues from 20 patients, aged 20-35 years, compared to endometrial tissues obtained from 20 normal women, age-matched to endometriosis group, who underwent micro-curettage to evaluate methylation status and mRNA expression of PGR-A and PGR-B gene. Methylation status of both PGRs was analyzed using MSP, and mRNA expression was investigated using qPCR. Methylation level of PGR-A (10,84%) and PGR-B (98,72%) in ovarian endometriosis were significantly different compared to control. The mRNA expression of PGR-A and PGR-B in ovarian endometriotic tissues significantly decreased by 2.35 and 6.37 folds compared to the normal endometrial tissues. Hypermethylation of PGR-B gene was correlated to mRNA expression, while methylation level of PGR-A had no correlation to mRNA expression. Promoter regions of PGR-A and -B genes in ovarian endometriosis are hypermethylated compared to controls. These hypermethylationmay reduce their expressions, hence might contribute to pathogenesis of endometriosis.
KW - DNA methylation
KW - Endometriosis
KW - MRNA
KW - PGR
UR - http://www.scopus.com/inward/record.url?scp=85069520702&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85069520702
SN - 1309-100X
VL - 12
SP - 242
EP - 246
JO - Journal of International Dental and Medical Research
JF - Journal of International Dental and Medical Research
IS - 1
ER -
