TY - JOUR
T1 - Promoted megakaryocytic differentiation of K562 cells through oxidative stress caused by near ultraviolet irradiation
AU - Nurhayati, Retno Wahyu
AU - Ojima, Yoshihiro
AU - Nomura, Naoki
AU - Taya, Masahito
N1 - Funding Information:
Acknowledgements. This research was in part supported by Grant-in-Aids for Scientific Researches (No. 25289295) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. It was also supported by the Multidisciplinary Research Laboratory System for Future Developments of the Graduate School of Engineering Science of Osaka University.
Publisher Copyright:
© 2014, Versita Warsaw and Springer-Verlag Wien.
PY - 2014/12/21
Y1 - 2014/12/21
N2 - Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.
AB - Reactive oxygen species (ROS) have been proven to be important activators for various cellular activities, including cell differentiation. Several reports showed the necessity of ROS during cell differentiation of the megakaryocytic (MK) lineage. In this study, we employed near ultraviolet (near-UV) irradiation to generate endogenous oxidative stress in an MK differentiation process of K562 cells with phorbol 12-myristate 13-acetate (PMA) induction. A significant increase in the intracellular ROS level was detected on day 1 after near-UV irradiation. In the initial stage of differentiation, a shifted fraction of G1 and G2 phase cells was obtained using near-UV irradiation, giving an increased percentage of G2 phase cells (up from 31.1 to 68.7%). The near-UV irradiation-induced upregulation of the p21 gene, which is a cell cycle inhibitor, suggested that the G2 phase cells were prevented from undergoing cell division. It was found that the percentage of high ploidy (8N and 16N) cells was enhanced significantly at the later stage of the K562 cell culture with near-UV irradiation. Moreover, time-lapse analysis showed that near-UV irradiation encouraged the expression of CD41, a specific surface marker of megakaryocytes. This is the first report that the elevated oxidative stress through the near-UV irradiation promoted the MK differentiation of PMA-induced K562 cells.
KW - Cell cycle
KW - Irradiation
KW - K562 cells
KW - Megakaryocytic differentiation
KW - Near ultraviolet
KW - Phorbol 12-myristate 13-acetate
KW - Polyploidization
KW - Reactive oxygen species
UR - http://www.scopus.com/inward/record.url?scp=84922091707&partnerID=8YFLogxK
U2 - 10.2478/s11658-014-0215-3
DO - 10.2478/s11658-014-0215-3
M3 - Article
C2 - 25338769
AN - SCOPUS:84922091707
SN - 1425-8153
VL - 19
SP - 590
EP - 600
JO - Cellular and Molecular Biology Letters
JF - Cellular and Molecular Biology Letters
IS - 4
ER -