HIV infection is one of global health problems. The number of new infection is increasing in Indonesia. This research aims to study the profile of HIV patients in rural area. This reasearch is a cross-sectional study on patients registers in Sehati Voluntary Counseling Testing / HIV care unit in Dr.TC Hillers District General Hospital, Sikka Regency during January untill December 2014 period. There were 104 subjects diagnosed as HIV positive and 64.4% of them were male. The most common education level was middle school (46.2%) and elementary school (44.2%). The most frequent transmission route is heterosexual intercourse (74%). The most usual symptoms suffered by subjects are coughing, weight loss, and fever. The most prevalent opportunistic infection are pulmonary tuberculosis and oral candidosis. HIV infection is a complex problem therefore it needs a holistic treatment. Education plays an essential role in improving sexual health literacy, preventing new infection and breaking the infection chain. Peer group support plays an important role in improving the quality of life HIV patients.