Based on research conducted by various researchers showing that sea cucumbers are high in nutrients needed by the body such as proteins, polysaccharides, fats, amino acids, and show anti-bacterial activity, anti-fungi and antioxidant which are good for the body. From the information above, supplement with sea cucumber-based ingredients can produce supplements that rich in nutrients. In this study, the authors has done the production of supplements with the main ingredients is sea cucumber that most common found in Indonesia which is Holothuria scabra in the same form as commercial products and has conducted a comparative test, composition identification and test of anti-bacterial, anti-fungi and antioxidant activity. The result of the composition obtained are jelly made from 35% hydrolysate, 45% water, 15% gelatin and 5% sugar. The results of sample jelly protein 5.1% and commercial jelly protein 0.175%, result of sample jelly fat 0.03% and commercial jelly fat 0.06%, and result of sample jelly carbohydrate 2.6% and commercial jelly carbohydrate 2.9%. Antioxidant and anti-bacterial tests also show that artificial sea cucumber jelly has a higher activity. It can be concluded that sea cucumber jelly has good nutrients and good antioxidant activity and good anti-microbial activity.