Production and Characterisation Partial Lipase of Bacillus halodurans Cml Mutant for Biodetergen

This study aims to produce lipase of the Bacillus halodurans CM1 mutant and its assess partialcharacteristics, performed in Bora and Bora modified medium. The purification was conducted usingUltrafiltration (UF), ammonium sulfate (AS) and polyethylene glycol (PEG). Results revealed that thehighest purity lipaseof B. halodurans CM1 mutant was 1.49-fold from the UF-AS-dyalisis, with a molecularweight of 35.7-37.4 KDa. The optimum condition of lipase enzyme was achieved at pH 7 and temperature50 °C, relatively stable at pH 7-8 and temperature 30-70 °C. Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ ions ofconcentrations, 1 mM to 10 mM increased enzyme lipase activity. The Km value was 0.23 mg/mL andVmax 4.07 U/mL. Lipase was stable with the addition of a detergent concentration of 1-2% (69.60-57.10%),and with the washing test, the enzyme capable of hydrolyzing oil on cloth is 8.40%.