TY - JOUR
T1 - Production and characterisation partial lipase of bacillus halodurans Cm1 mutant for biodetergen
AU - Aisyah, Arina
AU - Trismilah,
AU - Mangunwardoyo, Wibowo
AU - Suhendar, Dadang
N1 - Funding Information:
This research was supported by the Center for Bio-industrial Technology (PTB), Agency for the Assessment and Application of Technology (BPPT), which provided funding and laboratory facilities to conduct this study.
Publisher Copyright:
© 2021 PCSIR-Scientific Information Centre. All rights reserved.
PY - 2021
Y1 - 2021
N2 - This study aims to produce lipase of the Bacillus halodurans CM1 mutant and its assess partial characteristics, performed in Bora and Bora modified medium. The purification was conducted using Ultrafiltration (UF), ammonium sulfate (AS) and polyethylene glycol (PEG). Results revealed that the highest purity lipase of B. halodurans CM1 mutant was 1.49-fold from the UF-AS-dyalisis, with a molecular weight of 35.7-37.4 KDa. The optimum condition of lipase enzyme was achieved at pH 7 and temperature 50 °C, relatively stable at pH 7-8 and temperature 30-70 °C. Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ ions of concentrations, 1 mM to 10 mM increased enzyme lipase activity. The Km value was 0.23 mg/mL and Vmax 4.07 U/mL. Lipase was stable with the addition of a detergent concentration of 1-2% (69.60-57.10%), and with the washing test, the enzyme capable of hydrolyzing oil on cloth is 8.40%.
AB - This study aims to produce lipase of the Bacillus halodurans CM1 mutant and its assess partial characteristics, performed in Bora and Bora modified medium. The purification was conducted using Ultrafiltration (UF), ammonium sulfate (AS) and polyethylene glycol (PEG). Results revealed that the highest purity lipase of B. halodurans CM1 mutant was 1.49-fold from the UF-AS-dyalisis, with a molecular weight of 35.7-37.4 KDa. The optimum condition of lipase enzyme was achieved at pH 7 and temperature 50 °C, relatively stable at pH 7-8 and temperature 30-70 °C. Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ ions of concentrations, 1 mM to 10 mM increased enzyme lipase activity. The Km value was 0.23 mg/mL and Vmax 4.07 U/mL. Lipase was stable with the addition of a detergent concentration of 1-2% (69.60-57.10%), and with the washing test, the enzyme capable of hydrolyzing oil on cloth is 8.40%.
KW - B. halodurans
KW - Characterisation
KW - CM1 mutant
KW - Lipase
UR - http://www.scopus.com/inward/record.url?scp=85105687534&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85105687534
SN - 2221-6421
VL - 64
SP - 87
EP - 93
JO - Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences
JF - Pakistan Journal of Scientific and Industrial Research Series B: Biological Sciences
IS - 1
ER -