TY - JOUR
T1 - Prevalence of CYP2C19 681G>A and 636G>A Gene Polymorphisms in Javanese Farmers Exposed to Chlorpyrifos
AU - Fuk, Liem Jen
AU - Mansyur, Muchtaruddin
AU - Malik, Safarina G.
AU - Suryandari, Dwi A.
AU - Soemarko, Dewi S.
AU - Subekti, Imam
AU - Suyatna, Franciscus D.
AU - Kekalih, Aria
AU - Pangaribuan, Bertha
N1 - Funding Information:
The authors received financial support from Prodia Group. The funders had no role in the study's design, in the collection, analysis, and interpretation of data, or in the writing of the manuscript and the decision to publish.
Publisher Copyright:
© 2022 University of the Philippines Manila. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Objective. The objective of our study was to determine the genotype frequencies of CYP2C19*2 and *3 gene polymorphisms among Javanese farmers exposed to chlorpyrifos (CPF) in Central Java, Indonesia. Methods. This cross-sectional study was conducted from July to October 2020 in Central Java, Indonesia, involving 151 vegetable farmers aged 18-65 years who used CPF for at least one year. CYP2C19*2 and *3 gene polymorphisms were analyzed using PCR-RFLP. Direct calculations were applied to calculate allele and genotype frequencies. The difference in genotype frequencies among the sex and cumulative exposure level (CEL) group was performed using the Chi-square test. In contrast, the proportion difference of allele frequencies was analyzed using Z-test. Results. The frequency of CYP2C19 genotypes *1/*1, *1/*3, *1/*2, and *2/*2 were 64 %, 7 %, 23 % and 6 %. We observed no significant difference in the genotype distribution according to sex group and CEL group. Conclusion. In summary, the prevalence of toxicologically relevant CYP2C19 polymorphisms was determined in the Javanese agricultural population. The CYP2C19 genotype may be helpful as an essential biomarker of genetic susceptibility towards CPF exposure. Nevertheless, further studies to confirm the role of CYP2C19 in this context are still needed.
AB - Objective. The objective of our study was to determine the genotype frequencies of CYP2C19*2 and *3 gene polymorphisms among Javanese farmers exposed to chlorpyrifos (CPF) in Central Java, Indonesia. Methods. This cross-sectional study was conducted from July to October 2020 in Central Java, Indonesia, involving 151 vegetable farmers aged 18-65 years who used CPF for at least one year. CYP2C19*2 and *3 gene polymorphisms were analyzed using PCR-RFLP. Direct calculations were applied to calculate allele and genotype frequencies. The difference in genotype frequencies among the sex and cumulative exposure level (CEL) group was performed using the Chi-square test. In contrast, the proportion difference of allele frequencies was analyzed using Z-test. Results. The frequency of CYP2C19 genotypes *1/*1, *1/*3, *1/*2, and *2/*2 were 64 %, 7 %, 23 % and 6 %. We observed no significant difference in the genotype distribution according to sex group and CEL group. Conclusion. In summary, the prevalence of toxicologically relevant CYP2C19 polymorphisms was determined in the Javanese agricultural population. The CYP2C19 genotype may be helpful as an essential biomarker of genetic susceptibility towards CPF exposure. Nevertheless, further studies to confirm the role of CYP2C19 in this context are still needed.
KW - CYP2C19
KW - Farmer
KW - Pesticide
KW - Susceptibility biomarker
UR - http://www.scopus.com/inward/record.url?scp=85124902258&partnerID=8YFLogxK
U2 - 10.47895/amp.v56i1.4498
DO - 10.47895/amp.v56i1.4498
M3 - Article
AN - SCOPUS:85124902258
SN - 0001-6071
VL - 56
SP - 7
EP - 11
JO - Acta Medica Philippina
JF - Acta Medica Philippina
IS - 1
ER -