TY - GEN
T1 - Preparation of W/O/W double nanoemulsions loaded by red dragon fruit extract
AU - Harimurti, Niken
AU - Mulia, Kamarza
AU - Nasikin, M.
N1 - Funding Information:
This work was supported by Cooperation Partnership and National Agricultural Development Research program 2016 funded by Indonesian Agency for Agricultural Research and Development (IAARD), Indonesia Ministry of Agriculture.
Publisher Copyright:
© 2020 Author(s).
PY - 2020/9/3
Y1 - 2020/9/3
N2 - Betacyanin, the major compound in red dragon fruit extract is a high value bioactive polyphenol compound with wide application in functional food. This bioactive compound is very sensitive to oxidation and has low bioavaibility. In this research, we prepared w/o/w nanoemulsion encapsulating hydrophilic betacyanin using two-step high energy emulsification. Polyglycerol polyricinoleate (PGPR) as hydrophobic surfactant, soya bean oil, red dragon fruit extract, ultraturrax (10,000 rpm, 3'), ultrasonic (20 KHz, 5') were used for primary water in oil (w/o) emulsification. The influence of PGPR concentration and sonification time on the droplet size and Poly Dispersity Index (PDI) of the fabricated w/o primary emulsion was investigated. The results showed that PGPR concentration affected significantly on droplet size and PDI of w/o primary emulsion. The selected w/o primary emulsion (A3B3) was emulsificated with aqueous phase (20:80) and 1% w/w Tween 80 using High Pressure Homogenizer at 200 Bar, 3 cycles and formed w/o/w nanoemulsion with the following characteristics: droplet diameter was 199 nm; PDI was 0.18; zeta potential was-37.1 mV. The antioxidant activity of this w/o/w nanoemulsion applying the DPPH method was 9.15 ppm for betacyanin. It has been observed that betacyanin in nanoemulsion (0.068 %w/w) performed antioxidant activity 3.5% from betacyanin content in natural extract (0.34%). Hence, betacyanin loaded in w/o/w nanoemulsion was better as an antioxidant natural red dragon fruit extract.
AB - Betacyanin, the major compound in red dragon fruit extract is a high value bioactive polyphenol compound with wide application in functional food. This bioactive compound is very sensitive to oxidation and has low bioavaibility. In this research, we prepared w/o/w nanoemulsion encapsulating hydrophilic betacyanin using two-step high energy emulsification. Polyglycerol polyricinoleate (PGPR) as hydrophobic surfactant, soya bean oil, red dragon fruit extract, ultraturrax (10,000 rpm, 3'), ultrasonic (20 KHz, 5') were used for primary water in oil (w/o) emulsification. The influence of PGPR concentration and sonification time on the droplet size and Poly Dispersity Index (PDI) of the fabricated w/o primary emulsion was investigated. The results showed that PGPR concentration affected significantly on droplet size and PDI of w/o primary emulsion. The selected w/o primary emulsion (A3B3) was emulsificated with aqueous phase (20:80) and 1% w/w Tween 80 using High Pressure Homogenizer at 200 Bar, 3 cycles and formed w/o/w nanoemulsion with the following characteristics: droplet diameter was 199 nm; PDI was 0.18; zeta potential was-37.1 mV. The antioxidant activity of this w/o/w nanoemulsion applying the DPPH method was 9.15 ppm for betacyanin. It has been observed that betacyanin in nanoemulsion (0.068 %w/w) performed antioxidant activity 3.5% from betacyanin content in natural extract (0.34%). Hence, betacyanin loaded in w/o/w nanoemulsion was better as an antioxidant natural red dragon fruit extract.
UR - http://www.scopus.com/inward/record.url?scp=85092051960&partnerID=8YFLogxK
U2 - 10.1063/5.0013757
DO - 10.1063/5.0013757
M3 - Conference contribution
AN - SCOPUS:85092051960
T3 - AIP Conference Proceedings
BT - 4th International Tropical Renewable Energy Conference, i-TREC 2019
A2 - Kusrini, Eny
A2 - Nugraha, I. Gde Dharma
PB - American Institute of Physics Inc.
T2 - 4th International Tropical Renewable Energy Conference 2019, i-TREC 2019
Y2 - 14 August 2019 through 16 August 2019
ER -