Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

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Abstract

Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.

Original languageEnglish
Article number012006
JournalIOP Conference Series: Materials Science and Engineering
Volume188
Issue number1
DOIs
Publication statusPublished - 2 May 2017
EventInternational Symposium on Current Progress in Functional Materials 2016, ISCPFM 2016 - Bali, Indonesia
Duration: 26 Jul 201627 Jul 2016

Keywords

  • Acrylamide
  • Biosensor
  • Boron-Doped Diamond
  • Gold Nanoparticle
  • Haemoglobin

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