Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

K. Umam; Endang Saefudin; Ivandini Tribidasari Anggraningrum

doi:10.1088/1757-899X/188/1/012006

Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

K. Umam, Endang Saefudin, Ivandini Tribidasari Anggraningrum

Department of Chemistry

Research output: Contribution to journal › Conference article › peer-review

10 Citations (Scopus)

Abstract

Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.

Original language	English
Article number	012006
Journal	IOP Conference Series: Materials Science and Engineering
Volume	188
Issue number	1
DOIs	https://doi.org/10.1088/1757-899X/188/1/012006
Publication status	Published - 2 May 2017
Event	International Symposium on Current Progress in Functional Materials 2016, ISCPFM 2016 - Bali, Indonesia Duration: 26 Jul 2016 → 27 Jul 2016

Keywords

Acrylamide
Biosensor
Boron-Doped Diamond
Gold Nanoparticle
Haemoglobin

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10.1088/1757-899X/188/1/012006

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title = "Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors",

abstract = "Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.",

keywords = "Acrylamide, Biosensor, Boron-Doped Diamond, Gold Nanoparticle, Haemoglobin",

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T1 - Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

AU - Umam, K.

AU - Saefudin, Endang

AU - Anggraningrum, Ivandini Tribidasari

N1 - Publisher Copyright: © Published under licence by IOP Publishing Ltd.

PY - 2017/5/2

Y1 - 2017/5/2

N2 - Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.

AB - Boron-doped diamond (BDD) electrode was modified with haemoglobin to develop electrochemical biosensors of acrylamide. Prior to modify with haemoglobin, the BDD was modified by gold nanoparticles to increase the affinity of BDD against haemoglobin. The electrochemical behaviour of the electrode in the presence of acrylamide was studied in comparison to haemoglobin-modified gold electrodes. Cyclic voltammetry indicated the optimum responses in 0.1 M sodium acetate buffer at pH 5. The responses were linear to the acrylamide concentration range of 5-50 μM with an estimated detection limit of 5.14 μM, suggesting that the electrode was promising for acrylamide biosensors.

KW - Acrylamide

KW - Biosensor

KW - Boron-Doped Diamond

KW - Gold Nanoparticle

KW - Haemoglobin

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DO - 10.1088/1757-899X/188/1/012006

M3 - Conference article

AN - SCOPUS:85019671451

SN - 1757-8981

VL - 188

JO - IOP Conference Series: Materials Science and Engineering

JF - IOP Conference Series: Materials Science and Engineering

IS - 1

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T2 - International Symposium on Current Progress in Functional Materials 2016, ISCPFM 2016

Y2 - 26 July 2016 through 27 July 2016

ER -

Preparation of hemoglobin-modified boron-doped diamond for acrylamide biosensors

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Keywords

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