TY - JOUR
T1 - Preparation and stability evaluation of ll-37 cream
AU - Miranda, Eliza
AU - Bramono, Kusmarinah
AU - Rizky, Luddwi Achmad
AU - Hayun,
N1 - Funding Information:
The authors would like to express gratitude to the Directorate of Research?and?Community?Engagements?Universitas?Indonesia?for? the research grant Tugas Akhir Dosen (TADOK) 2019.
Funding Information:
The authors would like to express gratitude to the Directorate of Research and Community Engagements Universitas Indonesia for the research grant Tugas Akhir Dosen (TADOK) 2019.
Publisher Copyright:
© 2021 The Authors. Published by Innovare Academic Sciences Pvt Ltd.
PY - 2021/11/7
Y1 - 2021/11/7
N2 - Objective: The present study aimed to prepare LL-37 in a cream formulation (O/W emulsion) and evaluate its stability by determining the physical changes in the cream and concentration of LL-37 using validated high-performance liquid chromatography. Methods: The method was conducted at room temperature using a C18 column (5 µm × 250 mm × 4.6 mm) as a stationary phase, a mixture of 0.1% trifluoroacetic acid (TFA)/water (A) and 0.1% TFA/acetonitrile (B) (85:15) as the mobile phase, a flow rate of 1.0mL/min, an d photodiode array set at 228 nm as the detector. The method was validated in compliance with the Association of Official Analytical Chemists and International Conference on Harmonization guidelines. It demonstrated excellent linearity, accuracy, precision, specificity, the limit of detection, and limit of quantitation. Results: The chromatographic analysis indicated minimal degradation of LL-37 during the 12-week, with a predicted expiry time of 99 and 75 mo stored at 4 °C and 28 °C, respectively. Conclusion: LL-37 cream establishes good physical characteristics and stabilizes the active ingredient, especially at 4 °C and 28 °C storage. Therefore, the emulsion delivery system of LL-37 cream is harmless and stable as a novel alternative vehicle of LL-37.
AB - Objective: The present study aimed to prepare LL-37 in a cream formulation (O/W emulsion) and evaluate its stability by determining the physical changes in the cream and concentration of LL-37 using validated high-performance liquid chromatography. Methods: The method was conducted at room temperature using a C18 column (5 µm × 250 mm × 4.6 mm) as a stationary phase, a mixture of 0.1% trifluoroacetic acid (TFA)/water (A) and 0.1% TFA/acetonitrile (B) (85:15) as the mobile phase, a flow rate of 1.0mL/min, an d photodiode array set at 228 nm as the detector. The method was validated in compliance with the Association of Official Analytical Chemists and International Conference on Harmonization guidelines. It demonstrated excellent linearity, accuracy, precision, specificity, the limit of detection, and limit of quantitation. Results: The chromatographic analysis indicated minimal degradation of LL-37 during the 12-week, with a predicted expiry time of 99 and 75 mo stored at 4 °C and 28 °C, respectively. Conclusion: LL-37 cream establishes good physical characteristics and stabilizes the active ingredient, especially at 4 °C and 28 °C storage. Therefore, the emulsion delivery system of LL-37 cream is harmless and stable as a novel alternative vehicle of LL-37.
KW - Cream
KW - High-performance liquid chromatography
KW - LL-37
KW - Stability
UR - http://www.scopus.com/inward/record.url?scp=85121048883&partnerID=8YFLogxK
U2 - 10.22159/IJAP.2021V13I6.39639
DO - 10.22159/IJAP.2021V13I6.39639
M3 - Article
AN - SCOPUS:85121048883
SN - 0975-7058
VL - 13
SP - 139
EP - 143
JO - International Journal of Applied Pharmaceutics
JF - International Journal of Applied Pharmaceutics
IS - 6
ER -