Preparation and characteristics of nanostructured lipid carrier (NLC) loaded red ginger extract using high pressure homogenizer method

This study presents the preparation of nanostructured lipid carrier (NLC) loaded extract of Zingiber officinale. Roxc. Var rubrum or Red Ginger (RG-NLC) using High-Pressure Homogenizer method with different the number of cycles. The RG-NLC formulation was characterized concerning particle size, polydispersity index (PDI), zeta-potential, particle shape and entrapment efficiency. The morphological study was performed by using Zetasizer Nano S and the entrapment efficiency analysis of NLC was performed using HPLC by detecting [6]-, (8)-, (10)-gingerol and (6)-shogaol as active biomarkers. The average particle size for RG-NLCs ranged from size 131 to 154 nm and a polydispersity index of 0.104 to 0.172. The zeta potential of the RG-NLC differed −33.00 ± 1.57 to -46.53 ± 0.38 mV and transmission electron microscopy showed that the particles were spherical in shape. Entrapment efficiency was higher more than 98% for (6)-gingerol, but other active biomarkers were less than 96 %.