Practical isolation of bullatacin from Annona muricata leaves extract using an open column chromatography technique

Kamarza Mulia, Silvester W. Winarcahyo, Elsa Anisa Krisanti, Dewi Kurniasuci

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

Annonaceous acetogenins are bioactive compounds present in the leaves of Annona muricata (soursop) with significant anti-cancer activities. In this study, acetogenin-rich ethanol fraction extracted from soursop leaves was further separated and isolated using a simple open column chromatography technique with silica gel as the stationary phase. The separation procedure consists of three steps, each step employing sequential elution with organic solvents having different polarity as the eluent. Eluted fractions rich in acetogenin compounds were identified using the Kedde reagent which formed a distinctive dark green complex with the unsaturated γ-lactone group present in all of the annonaceous acetogenin compounds. HPLC analysis showed that the initial methanol fraction, as well as isolates from the second and the third isolation steps, contain bullatacin, squamocin, squamostatin-A, and squamostatin-D. Bullatacin produced from this study can be used further as a standard compound for quantitative analysis of other acetogenin compounds contained in soursop leaves extract.

Original languageEnglish
Title of host publicationAdvances in Materials, Processing and Manufacturing
Pages545-550
Number of pages6
DOIs
Publication statusPublished - 29 Oct 2013
Event13th International Conference on Quality in Research, QiR 2013 - Yogyakarta, Indonesia
Duration: 25 Jun 201328 Jun 2013

Publication series

NameAdvanced Materials Research
Volume789
ISSN (Print)1022-6680

Conference

Conference13th International Conference on Quality in Research, QiR 2013
Country/TerritoryIndonesia
CityYogyakarta
Period25/06/1328/06/13

Keywords

  • Annonaceous acetogenin
  • Bullatacin
  • Open column chromatography
  • Soursop

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