TY - GEN
T1 - Potential of Human Wharton's Jelly Mesenchymal Stem Cells (hWJMSCs) Secretome for COVID-19 Adjuvant Therapy Candidate
AU - Widowati, Wahyu
AU - Wargasetia, Teresa Liliana
AU - Rahardja, Fanny
AU - Gunanegara, Rimonta F.
AU - Handayani, Tri
AU - Kusuma, Hanna Sari Widya
AU - Arumwardana, Seila
AU - Wijayanti, Cahyaning Riski
AU - Rizal, Rizal
N1 - Funding Information:
The starving hWJMSCs-secretome (conditioned medium) were harvested and the hWJMSCs-secretome was measured for the IL-1ra level. The IL-1ra level of hWJMSCs-secretome is shown in Fig. 3. Based on the obtained results, the level of IL-1ra was increased for a We acknowledge and Thank you to theRpublicof Indonesia's Ministry of Education, Culture,Research,and Technology for the research grant 2021 (Penelitian Terapan Unggulan Perguruan Tinggi 2021). The studymethodan laboratory facilities were also provided by Aretha Medika Utama Biomolecular and Biomedical Research Centerin Bandung, Indonesia. We would like to express our gratitude toErviAfifah,MuhamadAldiMaulana,MeganitaMarthania of Aretha MedikaUtama,Biomolecular and Biomedical
Publisher Copyright:
© 2021 IEEE.
PY - 2021/7/14
Y1 - 2021/7/14
N2 - SARS-CoV-2 is a virus that causes a serious life-threatening disease known as COVID-19. Patients with COVID19 can develop acute respiratory distress syndrome (ARDS) and multiorgan failure requiring safe and effective therapy. The human Wharton's Jelly Mesenchymal Stem Cells (hWJMSCs) can act as an anti-inflammatory and regenerative properties due to their secretion such as cytokine, growth factor, antimicrobial, and chemokine. A natural way of producing the secreted proteins of a cell (secretome) is by culturing mesenchymal stem cells (MSCs) under starvation or serum deprivation condition. This research was performed to measure the level of Fibroblast Growth Factor-7 (FGF-7), antibacterial protein LL-37 (LL-37), interleukin 1 Receptor Antagonist (IL-1ra), respectively, in the secretome of hWJMSCs. The secretome levels at: non-starvation, 24-, 48-, and 72-hours starvation periods were measured using ELISA method. The hWJMSCs secreted levels of FGF-7, LL-37, and IL-1ra were 46.74 - 72.13 pg/mL, 1.70 - 4.14 ng/mL, 1.35 - 8.32 pg/mL, respectively. The longer the starvation period of cells, the higher the protein levels produced. The hWJMSCs secrete FGF-7, LL-37, IL-1ra, longer starvation periods of cells will increase secretome levels. The hWJMSCs could potentially serve as adjuvant therapy for COVID-19.
AB - SARS-CoV-2 is a virus that causes a serious life-threatening disease known as COVID-19. Patients with COVID19 can develop acute respiratory distress syndrome (ARDS) and multiorgan failure requiring safe and effective therapy. The human Wharton's Jelly Mesenchymal Stem Cells (hWJMSCs) can act as an anti-inflammatory and regenerative properties due to their secretion such as cytokine, growth factor, antimicrobial, and chemokine. A natural way of producing the secreted proteins of a cell (secretome) is by culturing mesenchymal stem cells (MSCs) under starvation or serum deprivation condition. This research was performed to measure the level of Fibroblast Growth Factor-7 (FGF-7), antibacterial protein LL-37 (LL-37), interleukin 1 Receptor Antagonist (IL-1ra), respectively, in the secretome of hWJMSCs. The secretome levels at: non-starvation, 24-, 48-, and 72-hours starvation periods were measured using ELISA method. The hWJMSCs secreted levels of FGF-7, LL-37, and IL-1ra were 46.74 - 72.13 pg/mL, 1.70 - 4.14 ng/mL, 1.35 - 8.32 pg/mL, respectively. The longer the starvation period of cells, the higher the protein levels produced. The hWJMSCs secrete FGF-7, LL-37, IL-1ra, longer starvation periods of cells will increase secretome levels. The hWJMSCs could potentially serve as adjuvant therapy for COVID-19.
KW - Acute Respiratory Distress Syndrome
KW - cytokine storm
KW - hWJMSCs
KW - SARS-CoV-2
UR - http://www.scopus.com/inward/record.url?scp=85115973165&partnerID=8YFLogxK
U2 - 10.1109/InHeNce52833.2021.9537290
DO - 10.1109/InHeNce52833.2021.9537290
M3 - Conference contribution
AN - SCOPUS:85115973165
T3 - InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences
BT - InHeNce 2021 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences
PB - Institute of Electrical and Electronics Engineers Inc.
T2 - 2021 IEEE International Conference on Health, Instrumentation and Measurement, and Natural Sciences, InHeNce 2021
Y2 - 14 July 2021 through 16 July 2021
ER -
